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Citation: YANG Shaoming, ZHA Wenling, SUN Qin, LI Hong, LI Ruiqin, SHANG Peiling. Label-free Elechochemical Aptasensor Based on Nickel Hexacyanoferrate for the Detection of Thrombin[J]. Chinese Journal of Applied Chemistry, ;2014, 31(6): 742-748. doi: 10.3724/SP.J.1095.2014.30416 shu

Label-free Elechochemical Aptasensor Based on Nickel Hexacyanoferrate for the Detection of Thrombin

1.
Department of Chemistry and Chemical Engineering, School of Sciences, East China Jiaotong University, Nanchang 330013, China

Corresponding author: YANG Shaoming,
Received Date: 16 August 2013
Available Online: 11 October 2013
Fund Project: 国家自然科学基金资助项目(21065004) (21065004)江西省教育厅科技项目(GJJ12304) (GJJ12304)

A label-free electrochemical aptasensor has been constructed for the electrochemical detection of thrombin.Two layers of nickel hexacyanoferrate redox probes sandwiched with multi-walled carbon nanotubes for the sensitivity enhancement were directly immobilized on the electrode surface by electrodeposition,and gold nanoparticles was used as the immobilization matrix for the aptamers.SEM was used to characterize the morphologies of multi-walled carbon nanotubes and nickel hexacyanoferrate.Electrochemical impedance spectroscopy was used to monitor the self-assembly process of aptamer biosensors.Electrochemical behaviors of aptamer biosensors were studied by cyclic voltammetry and differential pulse voltammetry.The biosensor shows a good linear range of 1.0 ng/L~1.0 mg/L(R=0.998) for the electrochemical detection of thrombin with a detection limit of 0.2 ng/L(S/N=3).
- nickel hexacyanoferrate,
- aptamer,
- thrombin,
- biosensor
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