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Citation: FAN Yanqun, XU Jianzhong, XU Xun, LIU Yijie, XU Chen. Simultaneous Determination of Glucuronic Acid and Glucuronolactone Using Ion-exclusion Chromatography[J]. Chinese Journal of Applied Chemistry, ;2014, 31(4): 444-449. doi: 10.3724/SP.J.1095.2014.30299 shu

Simultaneous Determination of Glucuronic Acid and Glucuronolactone Using Ion-exclusion Chromatography

FAN Yanqun ^a,b ,
XU Jianzhong ^b ,
XU Xun ^a,b ,
LIU Yijie ^c ,
XU Chen ^b,,

1.
a School of Life Science, Xiamen University, Xiamen 361005, China;
2.
b Third Institute of Oceanography, State Oceanic Administration, Xiamen 361102, China;
3.
c Fujian Medical University, Fuzhou 350108, China

Corresponding author: XU Chen,
Received Date: 17 June 2013
Available Online: 1 August 2013
Fund Project: 国家海洋公益性行业专项(201005022) (201005022)中国大洋矿产资源研究开发协会专项(DY125-15-T-01)资助 (DY125-15-T-01)

An analytical method was established for the simultaneous determination of glucuronic acid and glucuronolactone using ion-exclusion chromatography.The separation was performed on a Boston HC-B75 H⁺ column at 25℃ by HPLC equipped with an ultraviolet detector at the wavelength of 220 nm.The mobile phase is 5 mmol/L sulfuric acid with a flow rate of 0.6 mL/min.The quantification was performed by an external standard.The linear ranges for glucuronic acid and glucuronolactone are 10~1200 mg/L and 5~600 mg/L(r >0.9999),respectively.The detection and quantification limits for the two compounds are 0.1 mg/L and 0.3 mg/L,respectively.The standard deviations for glucuronic acid and glucuronolactone are 1.00% and 0.35%,respectively.The recovery ratios for glucuronic acid and glucuronolactone are 99.69% and 98.21%,respectively.This method is convenient,rapid,sensitive and suitable for the simultaneous determination of glucuronic acid and glucuronolactone.Temperature,pH and solvent affected the stability of glucuronic acid.
- ion-exclusion chromatography,
- glucuronic acid,
- glucuronolactone,
- stability
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