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Citation: SUN Yao, WU Chengke, LI Quanmin. Spectrophotometric Determination of Ceftazidime Using Sodium Nitroprusside[J]. Chinese Journal of Applied Chemistry, ;2012, 29(9): 1082-1086. doi: 10.3724/SP.J.1095.2012.00458 shu

Spectrophotometric Determination of Ceftazidime Using Sodium Nitroprusside

1.
Henan Key Laboratory for Environmental Pollution Control, College of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China

Corresponding author: LI Quanmin,
Received Date: 12 October 2011
Available Online: 7 December 2011
Fund Project: 河南省教育厅自然科学基金资助项目(2008B150011) (2008B150011)

A new method is proposed to determine ceftazidime using sodium nitroprusside.In an alkaline solution,a brown product is formed by the reaction of ceftazidime with sodium nitroprusside with the stoichiometric ratio of 1:2.The amount of ceftazidime can be determined owing to the absorbance of the colored product.Absorbency is linear with the concentration of ceftazidime in the range of 2.50~810 mg/L,and the regression equation is A=0.02704+0.00218ρ(mg/L) with a correlation coefficient of 0.9987.The apparent molar absorption coefficient and the detection limit are 1.2×10³ L/(mol·cm) and 1.38 mg/L,respectively.This method is simple and fast,the study indicated that the method could be applied to the determination of ceftazidime in injection with satisfactory results.
- sodium nitroprusside,
- ceftazidime,
- spectrophotometry
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