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Citation: ZENG Han, GONG Lanxin. Function of Chemical Sensor Based on the Immobilization of Laccase on the Composite of Chitosan-g-N-carboxymethyl-2-sulfo-4, 5-2H Imidazolinone and Multiwall Carbon Nanotubes[J]. Chinese Journal of Applied Chemistry, ;2012, 29(4): 462-469. doi: 10.3724/SP.J.1095.2012.00260 shu

Function of Chemical Sensor Based on the Immobilization of Laccase on the Composite of Chitosan-g-N-carboxymethyl-2-sulfo-4, 5-2H Imidazolinone and Multiwall Carbon Nanotubes

ZENG Han ^, ,
GONG Lanxin

1.
Chemistry and Chemical Engineering Academy, Xinjiang Normal University, Urumuqi 830054, China

Corresponding author: ZENG Han,
Received Date: 16 June 2011
Available Online: 3 August 2011

The matrix for laccase immobilization consisting of chitosan-g-N-carboxymethyl-2-sulfo-4,5-2H imidazolinone(CTS-g-N-CSIDZ) and multiwall carbon nanotubes(MWCNTs) was prepared through MWCNTs non-covalently functionalized with CTS-g-N-CSIDZ.Laccase immobilization on the composite carrier was achieved mainly by physical adsorption together with active centers in laccase complexing with ligands on the carrier and therefore the immobilized laccase could retain its original conformation of active centre to a great extent.On the glassy carbon electrode modified by the composite with adsorbed laccase was fabricated by a layer of composite composed with entrapped laccase attached to the surface of bare electrode.On the basis of determinoffing the loading of the composite matrix towards laccase,specific activity of immobilized laccase,stability,reusability and catalytic 2,6-dimethoxyphenol(DMP) oxidization dynamic parameters of the immobilized laccase on the composite matrix,function of this chemical sensor based on the electrode modified with the composite which accommodated laccase were examined(DMP as substrate).Results indicated this composite matrix for laccase entrapment had superiority of high enzyme loading amount(81.7 mg/g),improved laccase specific activity in immobilized state(1.33 U/mg).While the entrapped laccase in composite modified electrode as electrochemical sensor showed favorable affinity towards its substrate:DMP(Michaelis-Menten constant K_M is 0.0918 mmol/L),relatively higher sensitivity to DMP(3680 mA·L/mol),lower detection limit for DMP(3.3×10^-4 mmol/L),its relatively higher responsible selectivity,favorable reproducibility,reusability and excellent long-term stability were obtained.Such advantages make this laccase-based electrode one of promising candidates as amperometric electrochemical sensor for application in sensing the specific phenol-like compound selectively.
- laccase,
- multiwall carbon nano-tubes,
- chitosan,
- enzymatic dynamics,
- chemical sensor
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