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Citation: ZHANG Yaqiu. Spectrophotometric Determination of Ampicillin Sodium Using Ninhydrin as Chromogenic Agents[J]. Chinese Journal of Applied Chemistry, ;2012, 29(3): 316-319. doi: 10.3724/SP.J.1095.2012.00160 shu

Spectrophotometric Determination of Ampicillin Sodium Using Ninhydrin as Chromogenic Agents

ZHANG Yaqiu ^,

1.
Pharmaceutical College of Liaoning Medical University, Jinzhou 121001, China

Corresponding author: ZHANG Yaqiu,
Received Date: 19 April 2011
Available Online: 5 July 2011

The reaction between ampicillin sodium and ninhydrin was investigated by spectrophotometry.A simple and rapid method for the determination of ampicillin sodium has been optimized.The apparent molar absorptivity of complex is 3.72×10³ L/(mol·cm) at 564 nm.Beer's law could be followed in the range of 10~90 mg/L of ampicillin sodium with correlation coefficient of 0.998 1.The relative standard deviation is 1.5%(n=6) and the recovery was over 99.0%.
- ampicillin sodium,
- ninhydrin,
- spectrophotometry
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