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Citation: DENG Xiang, HUANG Xiaomei. A Biosensor for Hydrogen Peroxide Based on Hemoglobin Peroxidase Immobilized on Nano-Au/Chitosan-ZrO₂/gold Electrode[J]. Chinese Journal of Applied Chemistry, ;2011, 28(3): 349-354. doi: 10.3724/SP.J.1095.2011.00210 shu

A Biosensor for Hydrogen Peroxide Based on Hemoglobin Peroxidase Immobilized on Nano-Au/Chitosan-ZrO₂/gold Electrode

DENG Xiang ^, ,
HUANG Xiaomei

1.
Department of Chemistry and Chemical Engineering, Sichuan University of Arts and Science, Dazhou 635000

Corresponding author: DENG Xiang,
Received Date: 12 April 2010
Available Online: 24 June 2010
Fund Project: 四川省教育厅重点项目(10ZA143) (10ZA143)四川省教育厅青年基金项目(10ZB105) (10ZB105)

A new approach to measure with hemoglobin (Hb)/nano-Au/chitosan (CS) -ZrO₂/Au modified electrode was established. Zirconium dioxide(ZrO₂) was dispersed in the chitosan(CS) to form a stable CSZrO₂ composite. This composite was first coated on the surface of gold electrode to provide an amino group interface for assembling nano-Au, followed by the adsorption of Hb to form a bilayer of Hb/nano-Au. The Hb/nano-Au/CS-ZrO₂/gold electrode was used as hydrogen peroxide (H₂O₂) sensor. Cycling voltammetric and chronoamperometric characterizations reveal that the modified electrode exhibit electrocatalytic behavior to H₂O₂. The results confirmed that the electrode exhibited high sensitivity, fast response, and linear response in the range of 3.9×10^-6~1.8×10^-3 mol/L(R=0.9956) with the lowest detection limit of 5.6×10^-7 mol/L (S/N=3).
- zirconium dioxide,
- chitosan,
- nano-Au,
- hemoglobin
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通讯作者: 陈斌, bchen63@163.com

A Biosensor for Hydrogen Peroxide Based on Hemoglobin Peroxidase Immobilized on Nano-Au/Chitosan-ZrO₂/gold Electrode