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Citation: WEI Xu, HAO Qing-Li, LU Lu-De, WANG Xin, YANG Xu-Jie. Electrochemical Impedance Spectroscopy Study of SH-ssDNA Self-Assembling,Hybridization and Orientation on Thin-film Nana-gold Electrode[J]. Chinese Journal of Applied Chemistry, ;2010, 27(8): 970-977. doi: 10.3724/SP.J.1095.2010.90772 shu

Electrochemical Impedance Spectroscopy Study of SH-ssDNA Self-Assembling,Hybridization and Orientation on Thin-film Nana-gold Electrode

Materials Chemistry Laboratory, Nanjing University of Science & Technology, Nanjing 210094

Received Date: 12 November 2009
Revised Date: 30 December 2009

The self-assembling,hybridization and orientation of SH-ssDNA on the surface of thin-film nano-gold electrodes were characterized systematically by means of the electrochemical impedance technique. The effects of assembling time,concentration and the length of SH-ssDNA on its self-assembling were investigated. The R_ct and surface coverage reached the highest values when assembling time was 15 h. The influences of the concentration and length of SH-ssDNA and its hybridization method with complementary DNA on the hybridization reaction were also studied. The result shows the change of R_ct decreases with the increase of the SH-ssDNA concentration. The R_ct was increased by 16% when the concentration of SH-ssDNA was 5 μmol/L. The data simulation and analysis of impedance spectroscopy indicated that a unique self-assembled monolayer of SH-ssDNA was formed with an upright orientation on the nano-Au electrode surface. The result shows the hybridization efficiency is closely associated with the coverage of SH-ssDNA.
- electrochemical impedance spectroscopy,
- SH-ssDNA,
- self-assembling,
- hybridization,
- orientation
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