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Citation: LIU Jian, LIU Zhong-Fang, HU Xiao-Li, KONG Ling, LIU Shao-Pu. Determination of Chondroitin Sulfate A by Resonance Rayleigh Scattering and Resonance Non-linear Scattering with Proteins as Probes[J]. Chinese Journal of Applied Chemistry, ;2010, 27(7): 842-848. doi: 10.3724/SP.J.1095.2010.90649 shu

Determination of Chondroitin Sulfate A by Resonance Rayleigh Scattering and Resonance Non-linear Scattering with Proteins as Probes

School of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, Chongqing 400715

Received Date: 9 October 2009
Revised Date: 28 January 2010

In a pH 2.5~4.0 Britton-Robinson(BR) buffer medium,some proteins such as bovine serum albumin(BSA),Chymotrypsin(Chy) and α-amylase(α-Amy) can react with chondroitin sulfate A(CS) via electrostatic force,hydrogen bond force or/and hydrophobic force to form binding products. As a result,the resonance Rayleigh scattering(RRS),second-order scattering(SOS) and frequency doubling scattering(FDS) were enhanced greatly with new scattering spectra appeared. The increments of scattering intensity(△I) were proportional to the concentration of CS in certain ranges. The detection limits(3σ) of CS in Chy,BSA and α-Amy systems were 1.4,2.0 and 1.8 μg/L(RRS method),2.3,2.9 and 2.5 μg/L(SOS method),5.8,13.2 and 9.6 μg/L (FDS method),respectively. Among them,the RRS method exhibited the highest sensitivity and the Chy-CS system was more sensitive than others. The characteristics of the spectra and optimal conditions of RRS method were investigated. The effects of external substances were tested. The established methods have a good selectivity and can be applied to the determination of CS in eye drops with satisfactory results.
- proteins,
- chondroitin sulfate A,
- resonance Rayleigh scattering,
- second-order scattering,
- frequency doubling scattering
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