Citation: GUO Zi-Xuan, YANG Zhi-Qing, WAN Yi, LI Jing-Hong. Application Research Progress in Biosensing and Bioimaging Based on CRISPR-Cas System[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(5): 706-720. doi: 10.19756/j.issn.0253-3820.231041 shu

Application Research Progress in Biosensing and Bioimaging Based on CRISPR-Cas System

GUO Zi-Xuan ¹ ,
YANG Zhi-Qing ¹ ,
WAN Yi ^1,, ,
LI Jing-Hong ^2,,

1.
State Key Laboratory of Marine Resource Utilization in South China Sea, Marine College, Hainan University, Haikou 570228, China;
2.
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China

Corresponding author: WAN Yi, LI Jing-Hong,
Received Date: 10 February 2023
Revised Date: 29 March 2023

Fund Project: Supported by the National Natural Science Foundation of China (No. 41866002).

Clustered interspaced short palindromic repeat (CRISPR)-CRISPR associated (Cas) system has a unique ability to cleave target nucleic acids and non-specific single-stranded nucleic acids, and has become a vital tool for gene editing and gene diagnosis in recent years. Because of its high sensitivity, high specificity and the ability to recognize single nucleotide variation, CRISPR-Cas system has been widely used in novel nucleic acid detection biosensors. First of all, the composition and classification of the CRISPR-Cas system were introduced and the development and application process of the CRISPR-Cas system were summarized in this review. On this basis, the mechanism and construction process of biosensors based on different types of CRISPR-CAS systems were introduced, and the recent research progress of biosensors based on CRISPR-Cas system were summarized, including the applications in nucleic acid detection, protein detection and metal ion detection, as well as the applications of CRISPR-CAS systems in genotyping, gene mapping, nucleic acid mutation and other biological imaging, which would provide reference for the subsequent research related to CRISPR-Cas system.
- CRISPR-Cas system,
- Nucleic acid diagnostic,
- Microbiological detection,
- Biosensor,
- Nucleic acid amplification,
- Review
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