Citation: FANG Tai, WANG Qi-Wei, DU Yi, SHI Li-Li, LI Tao. DNA Logic Circuit based on pH-Sensitive DNA Quadruplex Fluorescent Ligand for H₂O₂ Detection[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(5): 901-910. doi: 10.19756/j.issn.0253-3820.221401 shu

DNA Logic Circuit based on pH-Sensitive DNA Quadruplex Fluorescent Ligand for H₂O₂ Detection

FANG Tai ¹ ,
WANG Qi-Wei ¹ ,
DU Yi ¹ ,
SHI Li-Li ² ,
LI Tao ^1,,

1.
School of Chemistry and Material Science, University of Science and Technology of China, Hefei 230026, China;
2.
School of Chemistry and Chemical Engineering, Anhui University, Hefei 230601, China

Corresponding author: LI Tao, tlitao@ustc.edu.cn
Received Date: 4 August 2022
Revised Date: 25 October 2022

Fund Project: Supported by the National Natural Science Foundation of China (Nos. 21904122, 22074133, 21874124) and the Anhui Provincial Natural Science Foundation (No. 2008085MB42).

Cancer is one of the main threats to human health. Due to the abnormal proliferation of cancer cells, the surface microenvironment of cancer cells is in a weakly acidic state (~pH 6.5), and the concentrations of K⁺ and hydrogen peroxide (H₂O₂) are also significantly higher than normal levels. These environmental characteristics are of great significance in specific diagnosis and treatment of cancer. In view of the above environmental factors, an acid-responsive G-quadruplex (G4) fluorescent ligand (PSNB) that was fluorescently lit under acidic conditions (~pK_a 5.2) was synthesized, and its pK_a increased to 7.0 after binding to G4, which could work normally in acidic microenvironment of cancer cells. The designed probe could undergo addition reaction with HSO₃^-, resulting in quenching of the fluorescence, and the fluorescence was restored after H₂O₂ oxidation. Based on this, a K⁺ and H⁺ logically controlled H₂O₂ sensing platform using PSNB and G4 was constructed. Under the optimal conditions, fluorescence recovery intensity showed a good linear relationship with concentration of H₂O₂ in the range of 1-1000 μmol/L with a detection limit of 1 μmol/L, exhibiting a high selectivity to H₂O₂. The concentration of intracellular H₂O₂ in MCF-7 cell lysate was measured to be about 331 μmol/L, which was consistent with the concentration range previously reported (0.1-1 mmol/L).
- Fluorescent probe,
- G-quadruplex,
- Logic gate,
- Hydrogen peroxide,
- Acid response
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沈阳化工大学材料科学与工程学院沈阳 110142

DNA Logic Circuit based on pH-Sensitive DNA Quadruplex Fluorescent Ligand for H2O2 Detection

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通讯作者: 陈斌, bchen63@163.com

DNA Logic Circuit based on pH-Sensitive DNA Quadruplex Fluorescent Ligand for H₂O₂ Detection