Citation: LI Ting-Ting, HE Yue-Yue, ZHANG Jing-Jing, QIN Fang-Ling, LI Cong, CHEN Bang, SHEN Ye-Hua. Proteomics and Organic Acid Metabolic Pathways in Amygdalus Pedunculata Pall Leaves at Different Growth Periods[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(1): 72-83. doi: 10.19756/j.issn.0253-3820.221323 shu

Proteomics and Organic Acid Metabolic Pathways in Amygdalus Pedunculata Pall Leaves at Different Growth Periods

1.
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China;
2.
School of Chemical Engineering, Northwest University, Xi'an 710069, China;
3.
College of Chemistry and Chemical Engineering, Xi'an Shiyou University, Xi'an 710065, China

Corresponding author: SHEN Ye-Hua, yhshen@nwu.edu.cn
Received Date: 30 June 2022
Revised Date: 25 September 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.21675125) and the Shaanxi Provincial Science and Technology Program (Nos.2018ZDXM-NY-087, 2019TSLNY03-02).

Based on proteomic analysis, the changes of protein species, content and metabolic pathway in the leaves of Amygdalus pedunculata Pall at different growth periods were investigated and clarified. The results showed that 6584 credible proteins were detected by tandem mass tags (TMT) labeled quantitative proteomics, and 1678 differential proteins were screened. The results of GO functional enrichment showed that the differential proteins participated in the biological processes such as defense response and response to biological stimulation, and mainly had molecular functions such as signal receptor activity, protein phosphatase inhibitor activity, glutathione transferase activity and glutathione dehydrogenase (ascorbic acid) activity.The content of organic acids in Amygdalus pedunculata Pall leaves decreased gradually from flowering stage to fruit ripening stage. Lyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that it was mainly related to α-linolenic acid metabolism, glyoxylic acid and dicarboxylic acid metabolism and carbon fixation in photosynthetic organisms. Traumatic acid, ketoglutarate, malic acid, citric acid and 12 related proteins played important roles in the metabolic process of organic acids in the leaves of Amygdalus pedunculata Pall at different growth stages.
- Amygdalus pedunculata Pall leaves,
- Proteomics,
- Metabolic pathway,
- Organic acid
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沈阳化工大学材料科学与工程学院沈阳 110142