Citation: LI Chang, XIE Jie, LIU Mei-Ying, HUANG Ze-Jian, DAI Xin-Hua, JIANG You, FANG Xiang, TIAN Di. Research on Simultaneous Fragmentation and Accumulation Technique Based on Quadrupole-Dual Pressure Linear Ion Trap Mass Spectrometer[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(2): 269-275. doi: 10.19756/j.issn.0253-3820.221315 shu

Research on Simultaneous Fragmentation and Accumulation Technique Based on Quadrupole-Dual Pressure Linear Ion Trap Mass Spectrometer

LI Chang ¹ ,
XIE Jie ^2,3 ,
LIU Mei-Ying ^2,3 ,
HUANG Ze-Jian ^2,3 ,
DAI Xin-Hua ² ,
JIANG You ^3,, ,
FANG Xiang ^2,, ,
TIAN Di ^1,,

1.
College of Instrumentation & Electrical Engineering, Jilin University, Changchun 130061, China;
2.
Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China;
3.
Technology Innovation Center of Mass Spectrometry for State Market Regulation, Beijing 100029, China

Corresponding author: JIANG You, FANG Xiang, TIAN Di,
Received Date: 27 June 2022
Revised Date: 27 July 2022

Fund Project: Supported by the National Key R&D Program of China (No. 2020YFF01014603), the National Natural Science Foundation of China (No. 21927812) and the Fundamental Research Funds of the National Institute of Metrology, China (No. AKY1934).

In this work, a quadrupole-dual pressure linear ion trap (Q-DPLIT) tandem mass spectrometer was developed and combined with a simultaneous technique to further improve the sensitivity and accuracy. The instrument consisted of a quadrupole and two hyperbolic ion traps in axial series. The precursor ions were filtered by the quadrupole into the linear ion trap Ⅰ (LITⅠ). At the same time, a composite waveform was applied to the LITI, so that the fragmentation of the precursor ions, isolation and accumulation of product ions occurred simultaneously. Then, the product ions were transferred to the linear ion trap Ⅱ (LITⅡ) for detection. By changing the helium flux introduced into LITⅠ, the ion trapping ability of the ion trap under different fluxes was investigated, and the optimal analysis condition was obtained. The results showed that the number of reserpine ions trapped by Q-DPLIT was 4.2 times as that of Q-LIT under the respective optimal conditions. The target peptide GVFGVF in concentrations ranging from 10 to 100 ng/mL with 100 μg/mL VVFFGG as the matrix was detected by Q-DPLIT. The mass-to-charge ratios (m/z) of VVFFGG and GVFGVF were both 625.3 amu. The ion accumulation time was 0.1-10 s. The signal intensity of the target product ion (m/z = 460.3 amu) measured by the simultaneous technique was improved by 1.3-10 times compared with that without this technique, and the linear correlation coefficient was increased from 0.6693-0.9449 to 0.9942-0.9994. The combination of Q-DPLIT and simultaneous technique could further improve the sensitivity and reduced the interferences of matrices. It was expected to achieve better analytical results in the analysis of trace substances in complex matrices in the future.
- Mass spectrometer,
- Quadrupole-dual pressure linear ion trap,
- Simultaneous fragmentation and accumulation technique,
- Isomers
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