Citation: WANG Xue, WANG An-Qi, QIAN Mei-Ru, LI Bin-Xi, JIN Long, LI Tai-Hua. Rapid and Highly Sensitive Detection of Methacycline Based on Ratiometric Fluorescent Probe and Sensitizer[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(4): 531-538. doi: 10.19756/j.issn.0253-3820.221311 shu

Rapid and Highly Sensitive Detection of Methacycline Based on Ratiometric Fluorescent Probe and Sensitizer

WANG Xue ^1,2 ,
WANG An-Qi ^1,2 ,
QIAN Mei-Ru ^1,2 ,
LI Bin-Xi ^1,2 ,
JIN Long ¹ ,
LI Tai-Hua ^1,2,,

1.
Co-Innovation Center for the Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China;
2.
National Positioning Observation Station of Hung-tse Lake Wetland Ecosystem in Jiangsu Province, Hongze 223100, China

Corresponding author: LI Tai-Hua, taehwali@njfu.edu.cn
Received Date: 23 June 2022
Revised Date: 8 December 2022

Fund Project: Supported by the Natural Science Research Project of Jiangsu Province, China (No. BK20191387).

Water soluble bovine serum albumin-gold nanoclusters (BSA-AuNCs) with good fluorescence properties were synthesized using BSA as template. According to the highly selective fluorescence response of methacycline (MTC) to BSA-AuNCs, a rapid and highly sensitive detection method for residual MTC in water was constructed on the basis of the highly selective fluorescence response of MTC to BSA-AuNCs and the fluorescence sensitization of non-ionic surfactant Tween 20. In the presence of MTC, the green fluorescence intensity at 525 nm was significantly increased and the red fluorescence intensity at 670 nm was slightly changed in comparison with BSA-AuNCs. Furthermore, it was observed that the ratiometric signal (F₅₂₅/F₆₇₀) of the detection system was obviously sensitized by non-ionic surfactants. Under the optimal experimental conditions, the linear concentration range of the system for MTC was 1-100 nmol/L, and the detection limit (LOD) was 0.71 nmol/L. In addition, the recoveries of MTC in the spiked water samples were 97%-108% with a relative standard deviations (RSD) lower than 10%. The developed fluorescent probe here could be used for simple, rapid, cost-effective, highly sensitive and selective detection of MTC in real applications.
- Methacycline,
- Tetracyclines,
- Ratiometric fluorescent probe,
- Bovine serum albumin-gold nanoclusters,
- Non-ionic surfactant
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