Citation: YU Shao-Nan, REN Ling-Ling, REN Li-Qun, BAI Ying-Jie, LIU Gui-Feng, ZHANG Hua, WANG Zhen-Xin. Upconversion Nanoparticles/Gold Nanorods-based Fluorescence Resonance Energy Transfer Immunoassay for Detection of Carcinoembryonic Antigen[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(9): 1299-1307. doi: 10.19756/j.issn.0253-3820.221249 shu

Upconversion Nanoparticles/Gold Nanorods-based Fluorescence Resonance Energy Transfer Immunoassay for Detection of Carcinoembryonic Antigen

1.
Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
2.
School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China;
3.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: LIU Gui-Feng, ZHANG Hua,
Received Date: 19 May 2022
Revised Date: 15 July 2022

Fund Project: Supported by the Wu Jieping Medical Foundation (Nos.320675019089-40, 320675019089-38) and the National Natural Science Foundation of China (No.61901438).

A fluorescence resonance energy transfer (FRET) immunoassay based on upconversion nanoparticles (UCNP) and gold nanorods (GNR) was constructed for sensitive detection of carcinoembryonic antigen (CEA). UCNP was employed as the donor and GNR was employed as the acceptor. The CEA antibody was covalently attached on the surface of UCNP and GNR to form UCNP-cAb and GNR-dAb conjugates, respectively. After introduction of CEA, a "sandwich" complex was formed through the antigen-antibody specific interaction, which resulted in the close proximity of UCNP and GNR to generate FRET. And the fluorescence quenching efficiency was positively correlated with the concentration of CEA. The constructed FRET immunoassay realized the detection of CEA in both buffer solution and human serum samples with a limit of detection (S/N=3) of 0.01 ng/mL, a linear range from 0.01 ng/mL to 100 ng/mL and good selectivity. It was expected that this FRET immunoassay could be further used in clinical applications and provided new methods for early diagnosis, treatment and prognosis monitoring of related cancers.
- Upconversion nanoparticles,
- Gold nanorods,
- Fluorescence resonance energy transfer,
- Carcinoembryonic antigen,
- Quantitative detection
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