Citation: WANG Jun-Yang, DUAN Zi-Xuan, WU Tong, WANG Wei, SUN Chun-Yan. Visual and Quantitative Detection of Lead Ion Based on G-quadruplex-hemin DNAzyme-mediated Etching of Gold Nanorods[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(10): 1473-1481. doi: 10.19756/j.issn.0253-3820.221180 shu

Visual and Quantitative Detection of Lead Ion Based on G-quadruplex-hemin DNAzyme-mediated Etching of Gold Nanorods

College of Food Science and Engineering, Jilin University, Changchun 130062, China

Corresponding author: SUN Chun-Yan, sunchuny@jlu.edu.cn
Received Date: 13 April 2022
Revised Date: 2 July 2022

Fund Project: Supported by the Innovation Training Project of Jilin University's "College Student Innovation and Entrepreneurship Training Program" (No.202110183164).

A label-free visualized lead ion biosensor was constructed based on G-quadruplex-hemin DNAzyme-mediated etching of gold nanorods (AuNRs). Hemin could induce the transformation of the aptamer PS2.M into a G-quadruplex structure to generate a G-quadruplex-hemin DNAzyme with peroxidase-like activity. 3,3',5,5'-Tetramethylbenzidine (TMB) was oxidized to form a blue diimine derivative TMB⁺, and the enzymatic reaction was terminated after adding H₂SO₄, and TMB⁺ became TMB²⁺ (yellow). Under the action of hexadecyl trimethyl ammonium bromide (CTAB), TMB²⁺ could effectively oxidize Au(0) to Au(I), thereby realizing the etching of AuNRs, and the solution turned yellow. Due to the highly specific interaction between PS2.M and Pb²⁺, and the ability of Pb²⁺ to stabilize G-quadruplexes was significantly stronger than that of hemin, G-quadruplexes-hemin DNAzyme with enzyme-like activity could not be formed in the presence of Pb²⁺, thus unable to etch AuNRs. Therefore, different concentrations of Pb²⁺ made the solutions produce distinct color changes that could be easily identified with naked eyes. The ratiometric quantitative detection of Pb²⁺ was achieved by measuring the absorbance of the longitudinal surface plasmon absorption peak of AuNRs and the TMB⁺ absorption peak at 450 nm, with a linear range of 5-5000 nmol/L and a detection limit as low as 2.0 nmol/L. The method was successfully applied to detection of Pb²⁺ in Songhua River water and tap water samples, with spiked recoveries were 98.7%-102.5% and 94.8%-108.2%, respectively, and the results were consistent with the detection results of inductively coupled plasma-mass spectrometry (ICP-MS). The method ingeniously designed the nucleic acid sequence, and realized the rapid and high-sensitivity visual detection of Pb²⁺.
- Aptamer,
- G-quadruplex-hemin DNAzyme,
- Lead ion,
- Gold nanorods,
- Visualization
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