Citation: LI Zhao-Fa, ZENG Jing-Yi, MAO Yu-Rui, FAN Ke-Xin, JIANG Min. Development of a Simple and Ultra-Sensitive Method for Rapid Detection of H1N1 Influenza A Virus[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(10): 1482-1490. doi: 10.19756/j.issn.0253-3820.221119 shu

Development of a Simple and Ultra-Sensitive Method for Rapid Detection of H1N1 Influenza A Virus

LI Zhao-Fa ^1,2,, ,
ZENG Jing-Yi ² ,
MAO Yu-Rui ² ,
FAN Ke-Xin ¹ ,
JIANG Min ¹

1.
School of Medicine, Huaqiao University, Quanzhou 362021, China;
2.
School of Biomedical Sciences, Huaqiao University, Quanzhou 362021, China

Corresponding author: LI Zhao-Fa, lizhaofa@hqu.edu.cn
Received Date: 10 March 2022
Revised Date: 6 July 2022

Fund Project: Supported by the Guidance Project of Fujian Province of China (No.2021Y0027) and the Project of Fujian Postgraduate Supervisor Team.

A rapid, simple and ultra-sensitive detection method for H1N1 influenza A virus (IAV) was developed based on aptamers and rolling circle amplification technique. Ten candidate aptamers of H1N1 IAV were screened by magnetic bead-systematic evolution of ligands by exponential enrichment (SELEX) technique, cloned, sequenced and combined repeat sequence. The binding force, specificity and affinity of aptamers were characterized by dot blot hybridization and enzyme linked immunosorbent assay (ELISA), and two linear aptamers Apt2 and Apt6 with good specificity and high affinity were obtained. H1N1 A influenza virus was captured and enriched by linear Apt2 modified on the surface of magnetic nanospheres. Then H1N1 IAV was combined with the circular aptamer CApt6, which was used as a template for rolling circle amplification (RCA). The detection results were displayed by gold nanoparticles visualization method. The results showed that the detection limit of H1N1 virus was 100.0 copies/μL. This work provided a new idea for development of ultra-sensitive and rapid method for detection of virus.
- H1N1 influenza A virus,
- Aptamer,
- Rolling circle amplification,
- Gold nanoparticles,
- Visual detection
1. [1]
  BAKKEN I J, AABERG K M, GHADERI S, GUNNES N, TROGSTAD L, MAGNUS P, HABERG S E. BMC Infectious Diseases, 2015, 15(1):506.
2. [2]
  TIALLA D, SAUSY A, CISSE A, SAGNA T, ILBOUDOA A K, OUEDRAOGO G A, HUBSCHEN J M, TARNAGAD Z, SNOECKC C J. Vet. Microbiol., 2020, 241:108572.
3. [3]
  TANG X, DU R H, WANG R, CAO T Z, GUAN L L, YANG C Q, ZHU Q, HU M, LI X Y, LI Y, LIANG L R, TONG Z H, SUN B, PENG P, SHI H Z. Chest, 2020, 158(1):195-205.
4. [4]
5. [5]
  COMIN A, TOFT N, STEGEMAN A, KLINKENBERG D, MARANGON S. Influenza Other Respir. Viruses, 2013, 7(3):257-264.
6. [6]
  GRABOWSKA I, MALECKA K, JAROCKA U, RADECKI J, RADECKA H. Acta Biochim. Pol., 2014, 61(3):471-478.
7. [7]
  NOTOMI T, OKAYAMA H, MASUBUCHI H, YONEKAWA T, WATANABE K, AMIAO N, HASE T. Nucleic Acids Res., 2000, 28(12):e63.
8. [8]
  NOTOMI T, MORI Y, TOMITA N, KANDA H. J. Microbiol., 2015, 53(1):1-5.
9. [9]
  FAMULOK M, MAYER G. Acc. Chem. Res., 2011, 44(12):1349-1358.
10. [10]
  TOWNSEND M B, DAWSON E D, MEHLMANN M, SMAGALA J A, DANKBAR D M, MOORE C L, SMITH C B, COX N J, KUCHTA R D, ROWLEN K L. J. Clin. Microbiol., 2006, 44(8):2863-2871.
11. [11]
  RAPINI R, MARRAZZA G. Bioelectrochemistry, 2017, 118:47-61.
12. [12]
13. [13]
  LAI H C, WANG C H, LIOU T M, LEE G B. Lab Chip, 2014, 14(12):2002-2013.
14. [14]
  BHARDWAJ J, CHAUDHARY N, KIM H, JANG J. Anal. Chim. Acta, 2019, 1064:94-103.
15. [15]
  GUPTA R, RAZA N, SANJEEV K, BHARDWA J, VIKRANT K, BHARDWAJ N. J. Hazard. Mater., 2021, 401:123379.
16. [16]
  WANG Y, LI H, WANG Y, LI H, LUO L, XU J, YE C. Int. J. Nanomed., 2017, 12:473-486.
17. [17]
18. [18]
  STOLTENBURG R, REINEMANN C, STREHLITZ B. Anal. Bioanal. Chem., 2005, 383(1):83-91.
19. [19]
  WU S J, LIU L H, DUAN N, LI Q, ZHOU Y, WANG Z P. J. Agric. Food Chem., 2018, 66(8):1949-1954.
20. [20]
  UZAWA T, TADA S, WANG W, ITO Y. Chem. Commun., 2013, 49(18):1786-1795.
21. [21]
1. [1]
  Xin Lv , Hongxing Zhang , Kaibo Duan , Wenhui Dai , Zhihui Wen , Wei Guo , Junsheng Hao . Lighting the Way Against Cancer: Photodynamic Therapy. University Chemistry, 2024, 39(5): 70-79. doi: 10.3866/PKU.DXHX202309090
2. [2]
  Zijuan LI , Xuan LÜ , Jiaojiao CHEN , Haiyang ZHAO , Shuo SUN , Zhiwu ZHANG , Jianlong ZHANG , Yanling MA , Jie LI , Zixian FENG , Jiahui LIU . Synthesis of visual fluorescence emission CdSe nanocrystals based on ligand regulation. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 308-320. doi: 10.11862/CJIC.20240138
3. [3]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
4. [4]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
5. [5]
  Hong RAO , Yang HU , Yicong MA , Chunxin LÜ , Wei ZHONG , Lihua DU . Synthesis and in vitro anticancer activity of phenanthroline-functionalized nitrogen heterocyclic carbene homo- and heterobimetallic silver/gold complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2429-2437. doi: 10.11862/CJIC.20240275
6. [6]
  Wenyan Dan , Weijie Li , Xiaogang Wang . The Technical Analysis of Visual Software ShelXle for Refinement of Small Molecular Crystal Structure. University Chemistry, 2024, 39(3): 63-69. doi: 10.3866/PKU.DXHX202302060
7. [7]
  Yanxin Wang , Hongjuan Wang , Yuren Shi , Yunxia Yang . Application of Python for Visualizing in Structural Chemistry Teaching. University Chemistry, 2024, 39(3): 108-117. doi: 10.3866/PKU.DXHX202306005
8. [8]
  Ruming Yuan , Laiying Zhang , Xiaoming Xu , Pingping Wu , Gang Fu . Application of Mathematica in Visualizing Physical Chemistry Formulas. University Chemistry, 2024, 39(8): 375-382. doi: 10.3866/PKU.DXHX202401030
9. [9]
  Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
10. [10]
  Guangming YIN , Huaiyao WANG , Jianhua ZHENG , Xinyue DONG , Jian LI , Yi'nan SUN , Yiming GAO , Bingbing WANG . Preparation and photocatalytic degradation performance of Ag/protonated g-C₃N₄ nanorod materials. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1491-1500. doi: 10.11862/CJIC.20240086
11. [11]
  Zhenli Sun , Ning Wang , Kexin Lin , Qin Dai , Yufei Zhou , Dandan Cao , Yanfeng Dang . Visual Analysis of Hotspots and Development Trends in Analytical Chemistry Education Reform. University Chemistry, 2024, 39(11): 57-64. doi: 10.12461/PKU.DXHX202403095
12. [12]
  Huiying Xu , Minghui Liang , Zhi Zhou , Hui Gao , Wei Yi . Application of Quantum Chemistry Computation and Visual Analysis in Teaching of Weak Interactions. University Chemistry, 2025, 40(3): 199-205. doi: 10.12461/PKU.DXHX202407011
13. [13]
  Jinghan ZHANG , Guanying CHEN . Progress in the application of rare-earth-doped upconversion nanoprobes in biological detection. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2335-2355. doi: 10.11862/CJIC.20240249
14. [14]
  Zhanhui Yang , Jiaxi Xu . (m+n+…) or [m+n+…]cycloaddition?. University Chemistry, 2025, 40(3): 387-389. doi: 10.12461/PKU.DXHX202406032
15. [15]
  Di Yang , Jiayi Wei , Hong Zhai , Xin Wang , Taiming Sun , Haole Song , Haiyan Wang . Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”. University Chemistry, 2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023
16. [16]
  Yongming Guo , Jie Li , Chaoyong Liu . Green Improvement and Educational Design in the Synthesis and Characterization of Silver Nanoparticles. University Chemistry, 2024, 39(3): 258-265. doi: 10.3866/PKU.DXHX202309057
17. [17]
  Hongyun Liu , Jiarun Li , Xinyi Li , Zhe Liu , Jiaxuan Li , Cong Xiao . Course Ideological and Political Design of a Comprehensive Chemistry Experiment: Constructing a Visual Molecular Logic System Based on Intelligent Hydrogel Film Electrodes. University Chemistry, 2024, 39(2): 227-233. doi: 10.3866/PKU.DXHX202309070
18. [18]
  Fugui XI , Du LI , Zhourui YAN , Hui WANG , Junyu XIANG , Zhiyun DONG . Functionalized zirconium metal-organic frameworks for the removal of tetracycline from water. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 683-694. doi: 10.11862/CJIC.20240291
19. [19]
  Junjie Zhang , Yue Wang , Qiuhan Wu , Ruquan Shen , Han Liu , Xinhua Duan . Preparation and Selective Separation of Lightweight Magnetic Molecularly Imprinted Polymers for Trace Tetracycline Detection in Milk. University Chemistry, 2024, 39(5): 251-257. doi: 10.3866/PKU.DXHX202311084
20. [20]
  Pengyang FAN , Shan FAN , Qinjin DAI , Xiaoying ZHENG , Wei DONG , Mengxue WANG , Xiaoxiao HUANG , Yong ZHANG . Preparation and performance of rich 1T-MoS₂ nanosheets for high-performance aqueous zinc ion battery cathode materials. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 675-682. doi: 10.11862/CJIC.20240339

Get Citation

PDF

XML

Metrics

PDF Downloads(12)
Abstract views(566)
HTML views(111)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142