Citation: YU Zi-Jing, XIAO Ming-Shu, PEI Hao, LI Li. DNA Origami-based Enzyme Cascade Catalysis for Electrochemical Detection of Low Density Lipoprotein[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(6): 850-858. doi: 10.19756/j.issn.0253-3820.221111 shu

DNA Origami-based Enzyme Cascade Catalysis for Electrochemical Detection of Low Density Lipoprotein

YU Zi-Jing ¹ ,
XIAO Ming-Shu ¹ ,
PEI Hao ¹ ,
LI Li ^1,2,,

1.
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China;
2.
Institute of Eco-Chongming, Shanghai 202162, China

Corresponding author: LI Li, lli@chem.ecnu.edu.cn
Received Date: 1 March 2022
Revised Date: 21 March 2022

Fund Project: Supported by the National Natural Science Foundation of China(Nos. 22074041, 22104038), the Science and Technology Commission of Shanghai Municipality(Nos. 22ZR1419800, 18490740500) and the China Postdoctoral Science Foundation(Nos. 2020TQ0097, 2021M701212).

The development of many diseases(e.g., atherosclerosis) is closely associated with abnormal level of low density lipoprotein(LDL), and developing methods for rapid and sensitive detection of LDL is of great importance to early diagnosis of atherosclerosis. In this work, a novel electrochemical sensor based on DNA origami-based enzymatic cascade catalysis was reported for detection of LDL. Benefiting from the programmability and addressability of nucleic acids, two enzymes(i.e., cholesterol oxidase and horseradish peroxidase) were specifically assembled on DNA origami, and the cascade catalysis efficiency towards LDL was optimized by regulating their distance, including 20, 40 and 80 nm. Through Au-S chemical bond, DNA origami loading enzymes was modified on Au electrode to construct enzymatic cascade catalysis-based electrochemical sensor. The results demonstrated that this sensor showed excellent performance for LDL detection, with a linear response range of 2-15 μmol/L, a low limit of detection of 1.8 μmol/L(S/N=3), and good selectivity. The recoveries of LDL spiked in healthy human serum sample were 97.8%-103.6%, indicating good reproducibility and stability. This electrochemical sensor utilizing DNA origami-based enzymatic cascade catalysis provided a promising tool for rapid and sensitive detection of LDL, holding a great potential for early diagnosis of atherosclerosis.
- DNA origami,
- Enzyme cascade catalysis,
- Electrochemical sensor,
- Low density lipoprotein
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