Citation: NIU Xiang-Heng, WANG Meng-Zhu, HU Pan-Wang, LIU Bang-Xiang. A Ratiometric Electroanalytical Method Based on Diazotization Reaction for Detection of Nitrite[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(6): 869-877. doi: 10.19756/j.issn.0253-3820.221083 shu

A Ratiometric Electroanalytical Method Based on Diazotization Reaction for Detection of Nitrite

1.
Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Normal University, Wuhu 241002, China;
2.
Institute of Green Chemistry and Chemical Technology, Jiangsu University, Zhenjiang 212013, China

Corresponding author: NIU Xiang-Heng, niuxiangheng@ujs.edu.cn
Received Date: 20 February 2022
Revised Date: 3 March 2022

Fund Project: Supported by the Key Laboratory of Functional Molecular Solids, Ministry of Education(No. FMS202001) and the National Natural Science Foundation of China(No. 21605061).

Excessive nitrite in food and water can cause many hazards to human health and the environment, therefore, it is very important to develop reliable, convenient and low-cost methods for nitrite detection. In this study, o-phenylenediamine(OPD) was used as a probe to develop a convenient ratiometric electrochemical method to realize high-performance detection of nitrite on bare screen-printed electrodes. The OPD probe could produce an oxidation signal at a low potential, and nitrite could produce another oxidation signal at a high potential. When OPD coexisted with nitrite, a diazotization reaction occurred in the acidic environment. With the increase of nitrite, free OPD decreased due to reaction consumption, resulting in the decreasing oxidation signal of the probe, while the oxidation signal of nitrite was gradually increased. Based on this principle, ratiometric electrochemical measurement of nitrite was achieved with a linear range of 10-300 μmol/L and a detection limit of 4.7 μmol/L. Accurate determination of the target in environmental water and pickle water samples was also demonstrated. The ratiometric electrochemical method with excellent sensitivity and accuracy, strong anti-interference ability, simple operation and low cost showed broad application prospects.
- Nitrite,
- Ratiometric detection,
- Electroanalysis,
- o-Phenylenediamine,
- Diazotization reaction
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