Citation: HUANG Xin, WANG Yi-Kai, XIU Yang, GUO Yun-Long, YUE Hao, CHEN Chang-Bao, LIU Shu-Ying. Analysis of Distribution of Neurochemicals in Rat Brain by Desorption Electrospray Ionization Time-of-Flight Mass Spectrometry Imaging[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(10): 1533-1541. doi: 10.19756/j.issn.0253-3820.221073 shu

Analysis of Distribution of Neurochemicals in Rat Brain by Desorption Electrospray Ionization Time-of-Flight Mass Spectrometry Imaging

HUANG Xin ^1,, ,
WANG Yi-Kai ¹ ,
XIU Yang ¹ ,
GUO Yun-Long ¹ ,
YUE Hao ^1,, ,
CHEN Chang-Bao ¹ ,
LIU Shu-Ying ^1,2

1.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China;
2.
Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: HUANG Xin, YUE Hao,
Received Date: 14 February 2022
Revised Date: 29 June 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.81903778) and the Science and Technology Development Plan Project of Jilin Province, China (No.20200201196JC).

The distribution of small molecular neurochemicals and their metabolites in rat brain was analyzed by desorption electrospray ionization time-of-flight mass spectrometry imaging (DESI-TOF-MSI) technique. The whole brain frozen horizontal sections of rats were prepared and scanned by DESI-TOF-MSI imaging. The influences of composition and proportion of spray solvents on the signal intensity of neurochemicals under positive and negative ion modes were also investigated. By comparison of high resolution mass spectrometry information in positive and negative ion mode and HMDB database searching, 25 kinds of neurochemicals and their metabolites were detected in rat brain. Under the condition of 0.1% formic acid-methanol spray, the molecular ion intensity of neurochemicals was increased mostly. HDImaging v1.5 was used for fine imaging analysis. Addition of 0.1% formic acid to the spray solvent could improve the imaging effect similar to the increasing trend of mass signal intensity. The method presented high repeatability. γ-Aminobutyric acid and aspartic acid were mainly distributed in cerebral cortex. Serine was detected throughout the brain. Glutamine was distributed around the hippocampus. The same spatial distribution of neurochemicals and their precursors and major metabolites in the brain was detected. The DESI-TOF-MSI technique developed here could be used to analyze the distribution of small molecular neurochemicals in rat brain. This work provided a fast and effective visualization method for the study of spatial distribution and metabolism of small molecule neurochemicals in brain.
- Desorption electrospray ionization time-of-flight mass spectrometry imaging,
- Neurochemicals,
- Brain,
- Distribution
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