Citation: WANG Ying-Fei, MENG Xiao, JU Huang-Xian, LIU Ying. Self-patterned Droplet Aptasensor for Electrochemical Detection of Human Interferon-gamma[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(11): 1743-1749. doi: 10.19756/j.issn.0253-3820.221059 shu

Self-patterned Droplet Aptasensor for Electrochemical Detection of Human Interferon-gamma

1.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China;
2.
Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing 210023, China

Corresponding author: LIU Ying, yingliu@nju.edu.cn
Received Date: 29 January 2022
Revised Date: 28 May 2022

Fund Project: Supported by the National Natural Science Foundation of China (Nos.22022405, 21974064).

An electrochemical aptasensor for analysis of human interferon-gamma (hIFN-γ) based on self-patterned droplet on substrate that is particularly suitable for point-of-care testing is reported. After fabricating the gold electrode system on glass slide via photolithography, the glass slide is functionalized with alkyne-silane. Sequential thiol-yne photoclick reactions are subsequently performed to develop hydrophilic 1-thioglycerol microwells around the electrodes with the surrounding regions modified with hydrophobic 1H,1H,2H,2H-perfluorodecanethiol. The hydrophilic microwells around electrode facilitate sample loading and enrichment by concentrating large-volume sample solution into small self-patterned droplet. The working electrode is further functionalized with thiolated aptamer for hIFN-γ. The hairpin structured aptamer molecule contains redox reporter molecule methylene blue, and the binding of analyte to aptamer-modified electrode inhibits electron transfer from redox reporters to the electrode and causes electrochemical redox signal decrease. The aptasensor demonstrates a linear relationship for hIFN-γ proteins from 10.0 ng/mL to 125.0 ng/mL with detection limit (3σ) of 7.5 ng/mL. The self-patterned droplet aptasensor developed here provides a convenient method for rapid cytokine hIFN-γ detection with high efficiency and accuracy, and demonstrates great potential for the rapid diagnosis of related diseases in a point-of-care testing manner.
- Cytokine,
- Aptasensor,
- Aptamer,
- Hydrophilic,
- Hydrophobic
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沈阳化工大学材料科学与工程学院沈阳 110142