Citation: ZHAO Chuang, LI Jia-Nan, SUN Xiu-Xia, XIAO Jian-Xi. A Novel Fluorescent Peptide Probe for Specific Detection of Gadolinium Ion(Ⅲ) in Cells and Zebrafish[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(7): 1014-1021. doi: 10.19756/j.issn.0253-3820.221056 shu

A Novel Fluorescent Peptide Probe for Specific Detection of Gadolinium Ion(Ⅲ) in Cells and Zebrafish

1.
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;
2.
School of Pharmacy, Lanzhou University, Lanzhou 730000, China

Corresponding author: SUN Xiu-Xia, XIAO Jian-Xi,
Received Date: 28 January 2022
Revised Date: 24 April 2022

Fund Project: Supported by the National Natural Science Foundation of China (Nos.22074057, 21775059).

As the most widely used magnetic resonance imaging contrast in clinical applications, gadolinium-based contrast agents play an important role in the clinical diagnosis and related research, while gadolinium deposition has been found to be closely related to nephrogenic systemic fibrosis and may cause multiple sclerosis, neurofibromatosis, inborn error of metabolism and other diseases. Therefore, the development of efficient assays for free Gd³⁺ is critical for the mechanistic study of these diseases. In this work, the fluorescent peptide probe PT consisted of (TPE-Glu-Glu-Glu-Pro-Gly-Glu-Glu-Glu) sequences was reported. The results showed that the efficient detection of Gd³⁺ in solution and biological fluids using the TPE-modified peptide probe PT without interferences of other metal ions was achieved. Meanwhile, the Job's analysis result indicated that the probe PT and Gd³⁺ formed a 1:3 complex and recovered its fluorescence, with a binding constant of 2.34×10¹⁵ L³/mol³. It was demonstrated that the fluorescent peptide probe PT provided a "Turn-On" sensing mode for Gd³⁺ with detection limits of 39 and 53 nmol/L in HEPES buffer solution and saliva samples. The probe PT was successfully used for imaging of Gd³⁺ in L929 cells and zebrafish. Notably, the newly developed fluorescent peptide probe PT had extraordinary features such as highly water solubility, low biotoxicity and high specificity for Gd³⁺ in zebrafish and L929 cells, which showed great potential in the molecular diagnosis of the concentration of residual Gd³⁺ in human body.
- Fluorescent peptide probe,
- Gadolinium ion(Ⅲ),
- L929 cells,
- Zebrafish
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沈阳化工大学材料科学与工程学院沈阳 110142