Citation: LIU Jia-Li, DENG Yan, LI Qiu-Xia, GUAN Yan, YANG Tong, MENG Shuang, YANG Yun-Hui, HU RONG. Development of DNA Sensor Based on Tetrahedral DNA Nanomaterials[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(9): 1308-1318. doi: 10.19756/j.issn.0253-3820.221024 shu

Development of DNA Sensor Based on Tetrahedral DNA Nanomaterials

Faculty of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China

Corresponding author: YANG Yun-Hui, HU RONG,
Received Date: 12 January 2022
Revised Date: 22 June 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.21765026).

Tetrahedral DNA nanomaterial (TDN) is a simple and strong pyramidal three-dimensional structure model. It has the advantages such as superior mechanical properties, stable structure, simple synthesis method, high synthesis yield, and unique specific recognition performance. In this work, a TDN-based electrochemical sensing platform was constructed for highly sensitive detection of DNA. By using Fe-metal organic framework (Fe-MOF) as signal probe, the detection did not require acid treatment and pretreatment process, which could save detection time. In addition, Fe-MOF was easy to functionalize. After the surface was loaded with precious metal nanoparticles, it was easy to modify nucleic acid or protein molecules, which could simplify the modification process. By using graphene-nano-gold particle composite materials with excellent performance as the substrate, it could further improve the sensitivity. When the target DNA was present, the purpose of detecting DNA was achieved by detecting the signal peak in the Fe-MOF material. In the concentration range of 0.01-140 pmol/L, the linear equation was I=2.858 × lgc(pmol/L)+6.488 (R²=0.9935), and the detection limit (3σ) was 4.5 fmol/L.
- Tetrahedral DNA nanomaterials,
- Fe-metal organic framework,
- DNA sensor
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