Citation: YANG Zhan, XU Bin, CHEN Jia, WU Jian-Feng, HE Yue-Zhong, XIE Jian-Wei. Identification of Some Novel Damage Biomarkers of Sulfur Mustard by High Resolution Mass Spectrometry in Vitro[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(10): 1733-1742. doi: 10.19756/j.issn.0253-3820.211256 shu

Identification of Some Novel Damage Biomarkers of Sulfur Mustard by High Resolution Mass Spectrometry in Vitro

YANG Zhan ^1,2 ,
XU Bin ² ,
CHEN Jia ² ,
WU Jian-Feng ^2,, ,
HE Yue-Zhong ¹ ,
XIE Jian-Wei ²

1.
Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China;
2.
State Key Laboratory of Toxicology and Medical Countermeasures and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China

Corresponding author: WU Jian-Feng, ammswjf@163.com
Received Date: 28 March 2021
Revised Date: 2 June 2021

Fund Project: Supported by the National Key Research and Development Program of China(Nos.2018YFC1602600, 2020YFF0305000).

Sulfur mustard (SM) is a typically representative alkylating agent with high reactivity. SM can produce a variety of hydrolytic and oxidative metabolites including various proteins and nucleic acid adducts. Among them, divinyl sulfone (DVS) is an important oxidative metabolite that should be paid more attention, which has high reactivity and toxicity close to SM. In this study, the human serum albumin (HSA) and human plasma were exposed to DVS and its two-phase metabolite DVS-GSH, respectively. After digestion with proteinase K and purification by solid phase extraction (SPE), UPLC-Q-extractive orbitrap high rosolution mass spectrometry (HRMS) was used to identify some albumin adducts. According to the analysis results, three novel biomarkers, DVS-Cys-Pro-Phe, Phe-Pro-Cys-DVS-Cys-Pro-Phe and GSH-DVS-Cys-Pro-Phe, were successfully identified by UPLC-Q-exactive orbitrap/HRMS. The results showed that both DVS and DVS-GSH could react with Cys-34 of albumin due to their highly reactive alkene bonds. These novel damage biomarkers not only provided new clues for SM exposure and diagnosis, but also provided evidence for elucidating the damage mechanism of SM from a new perspective.
- Sulfur mustard,
- Divinyl sulfone,
- Albumin adducts,
- Ultra performance liquid chromatography-tandem mass spectrometry
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