Citation: YANG Yang, GAO Shou-Hong, ZHANG Feng, ZHONG Ren-Qian, WANG Zhi-Peng, CHEN Wan-Sheng. Simultaneous Determination of 32 Kinds of Amino Acids in Plasma from Colorectal Cancer Patients Based on Targeted Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(7): 1083-1092. doi: 10.19756/j.issn.0253-3820.211255 shu

Simultaneous Determination of 32 Kinds of Amino Acids in Plasma from Colorectal Cancer Patients Based on Targeted Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
Department of Pharmacy, the Affiliated Huaihai Hospital of Xuzhou Medical University, the 71st Group Army Hospital of CPLA Army, Xuzhou 221004, China;
2.
Department of Pharmacy, Medical Service Center, the Affiliated Changzheng Hospital, Naval Medical University of CPLA, Shanghai 200003, China;
3.
Department of Clinical Laboratory Diagnostics, the Affiliated Changzheng Hospital, Naval Medical University of CPLA, Shanghai 200003, China

Corresponding author: WANG Zhi-Peng, CHEN Wan-Sheng,
Received Date: 27 March 2021
Revised Date: 31 March 2022

Fund Project: Supported by the International Science and Technology Cooperation and Communication Special Fund of China (No.2015DFA31810), the Scientific Research Fund of Shanghai Science and Technology Committee (No.17411972400), the Science and Technology Talent Development Project of CPLA Army, and the Xuzhou Medical Young Talent Project.

A targeted ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for simultaneous determination of 32 kinds of amino acids in plasma from colorectal cancer (CRC) patients. The chromatographic separation was performed on an Agilent Zorbax SB-C₁₈ column (150 mm×3.0 mm, 5 μm) with a binary gradient elution system (Mobile phase A:0.02% heptafluorobutyric acid and 0.2% formic acid in water; mobile phase B:methanol). The run time was 10 min. The multiple reaction monitoring mode was chosen with an electrospray ionization source operating in the positive ionization mode for data acquisition. The sample was pretreated based on protein precipitation. The average recoveries and matrix effects for 32 kinds of amino acids and 3 kinds of internal standards were 50.4%-182.4% and 41.0%-136.4%, respectively. The linear correlation coefficients were above 0.99 for 32 kinds amino acids. The intra- and inter-day accuracy for 32 kinds of amino acids ranged from -14.3% to 13.7% (RSD ≤ 8.8%) and from -14.4% to 13.4% (RSD ≤ 12.2%), respectively, and the deviations of stability under different conditions were within ±15%. This method was successfully utilized to quantify 32 kinds of amino acids in plasma samples from 6 CRC patients simultaneously.
- Amino acid,
- Colorectal cancer,
- Plasma,
- Ultra-high performance liquid chromatography-tandem mass spectrometry
1. [1]
  FERLAY J, COLOMBET M, SOERJOMATARAM I, PARKIN D M, PIÑEROS M, ZNAOR A, BRAY F. Int. J. Cancer, 2021, 149(4):778-789.
2. [2]
  LIU S Z, ZHENG R S, ZHANG M, ZHANG S W, SUN X B, CHEN W Q. Chin. J. Cancer Res., 2015, 27(1):22-28.
3. [3]
4. [4]
  BEZABEH T, SOMORJAI R, DOLENKO B, BRYSKINA N, LEVIN B, BERNSTEIN C N, JEYARAJAH E, STEINHART A H, RUBIN D T, SMITH I C.NMR Biomed., 2009, 22(6):593-600.
5. [5]
  FLETCHER R H. Ann. Intern. Med., 1986, 104(1):66-73.
6. [6]
  KRONBORG O, FENGER C, OLSEN J, JØRGENSEN O D, SØNDERGAARD O. Lancet, 1996, 348(9040):1467-1471.
7. [7]
  QIU Y P, CAI G X, SU M M, CHEN T L, LIU Y M, XU Y, NI Y, ZHAO A H, CAI S J, XU L X, JIA W. J. Proteome Res., 2010, 9(3):1627-1634.
8. [8]
  PINSKY P F, SCHOEN R E. JAMA Intern. Med., 2015, 175(5):858-860.
9. [9]
  HANAHAN D, WEINBERG R A. Cell, 2000, 100(1):57-70.
10. [10]
  HENRY C M. Chem. Eng. News, 2002, 80(48):66-70.
11. [11]
  NI Y, XIE G X, JIA W. J. Proteome Res., 2014, 13(9):3857-3870.
12. [12]
  NISHIUMI S, KOBAYASHI T, IKEDA A, YOSHIE T, KIBI M, IZUMI Y, OKUNO T, HAYASHI N, KAWANO S, TAKENAWA T, AZUMA T, YOSHIDA M. PLoS One, 2012, 7(7):e40459.
13. [13]
  ZHU J J, DENG L L, GU H W, HIMMATI F, CHIOREAN E G, RAFTERY D. J. Proteome Res., 2014, 13(9):4120-4130.
14. [14]
  KLEPACKI J, KLAWITTER J, KLAWITTER J, KARIMPOUR-FARD A, THURMAN J, INGLE G, PATEL D, CHRISTIANS U. Clin. Biochem., 2016, 49(13-14):955-961.
15. [15]
16. [16]
17. [17]
  YANG Z L, LI H, WANG B, LIU S Y. J. Chromatogr. B, 2016, 1012-1013:79-88.
18. [18]
  CALDERÓN-SANTIAGO M, PRIEGO-CAPOTE F, GALACHE-OSUNA J G, DE CASTRO M D L. J. Pharmaceut. Biomed. Anal., 2012, 70:476-484.
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
  WANG Q H, WEN Y, XIA T Y, XIONG X J, GAO S H, YOU C H, TAO X, ZHANG F, CHEN W S. Bioanalysis, 2016, 8(13):1337-1351.
24. [24]
  XIA T Y, FU S X, WANG Q H, WEN Y, CHAN S A, ZHU S, GAO S H, TAO X, ZHANG F, CHEN W S. Biomed. Chromatogr., 2018, 32(5):e4198.
25. [25]
  YANG Y, ZHANG F, GAO S H, WANG Z P, LI M M, WEI H, ZHONG R Q, CHEN W S. J. Anal. Methods Chem., 2020, 2020:4641709.
26. [26]
  VAN DE MERBEL N C. TrAC-Trends Anal. Chem., 2008, 27(10):924-933.
27. [27]
  ZHANG S, JIAN W Y, SULLIVAN S, SANKARAN B, EDOM R W, WENG N D, SHARKEY D. J. Chromatogr. B, 2014, 961(2):62-70.
28. [28]
  SUGIMOTO H, KAKEHI M, JINNO F. Anal. Biochem., 2015, 487(14):38-44.
29. [29]
  FURTADO D Z S, DE MOURA LEITE F B V, BARRETO C N, FARIA B, JEDLICKA L D L, SILVA E J, SILVA H D T, BECHARA E J H, ASSUNÇÃO N A. J. Pharmaceut. Biomed., 2017, 140:137-145.
30. [30]
  ROY C, TREMBLAY P Y, BIENVENU J F, AYOTTE P. J. Chromatogr. B, 2016, 1027:40-49.
31. [31]
  VISWANATHAN C T, BANSAL S, BOOTH B, DESTEFANO A J, ROSE M J, SAILSTAD J, SHAH V P, SKELLY J P, SWANN P G, WEINER R. AAPS J., 2007, 9(1):E30-E42.
32. [32]
33. [33]
  MATUSZEWSKI B K, CONSTANZER M L, CHAVEZ-ENG C M. Anal. Chem., 2003, 75(13):3019-3030.
1. [1]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
2. [2]
  Hong CAI , Jiewen WU , Jingyun LI , Lixian CHEN , Siqi XIAO , Dan LI . Synthesis of a zinc-cobalt bimetallic adenine metal-organic framework for the recognition of sulfur-containing amino acids. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 114-122. doi: 10.11862/CJIC.20240382
3. [3]
  Qian Wu , Yuanxia Lv , Zixuan Guo , Zhihao Zhao , Zhimin Zhang , Hongmei Lu . A Case Study and Practice of Research-Oriented Comprehensive Instrumental Analysis Laboratory Courses. University Chemistry, 2025, 40(10): 194-202. doi: 10.12461/PKU.DXHX202411063
4. [4]
  Ruiying WANG , Hui WANG , Fenglan CHAI , Zhinan ZUO , Benlai WU . Three-dimensional homochiral Eu(Ⅲ) coordination polymer and its amino acid configuration recognition. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 877-884. doi: 10.11862/CJIC.20250052
5. [5]
  Mengyang LI , Hao XU , Zhonghao NIU , Chunhua GONG , Weihui ZHONG , Jingli XIE . Highly effective catalytic synthesis of β-amino alcohols by using viologen-polyoxometalate hybrid materials. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1294-1300. doi: 10.11862/CJIC.20250080
6. [6]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
7. [7]
  Yu Peng , Jiawei Chen , Yue Yin , Yongjie Cao , Mochou Liao , Congxiao Wang , Xiaoli Dong , Yongyao Xia . Tailored cathode electrolyte interphase via ethylene carbonate-free electrolytes enabling stable and wide-temperature operation of high-voltage LiCoO₂. Acta Physico-Chimica Sinica, 2025, 41(8): 100087-0. doi: 10.1016/j.actphy.2025.100087
8. [8]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
9. [9]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
10. [10]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
11. [11]
  Ran Yu , Chen Hu , Ruili Guo , Ruonan Liu , Lixing Xia , Cenyu Yang , Jianglan Shui . Catalytic Effect of H₃PW₁₂O₄₀ on Hydrogen Storage of MgH₂. Acta Physico-Chimica Sinica, 2025, 41(1): 100001-0. doi: 10.3866/PKU.WHXB202308032
12. [12]
  Jiahe LIU , Gan TANG , Kai CHEN , Mingda ZHANG . Effect of low-temperature electrolyte additives on low-temperature performance of lithium cobaltate batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 719-728. doi: 10.11862/CJIC.20250023
13. [13]
  Ke Li , Chuang Liu , Jingping Li , Guohong Wang , Kai Wang . Architecting Inorganic/Organic S-Scheme Heterojunction of Bi₄Ti₃O₁₂ Coupling with g-C₃N₄ for Photocatalytic H₂O₂ Production from Pure Water. Acta Physico-Chimica Sinica, 2024, 40(11): 2403009-0. doi: 10.3866/PKU.WHXB202403009
14. [14]
  Guoqiang Chen , Zixuan Zheng , Wei Zhong , Guohong Wang , Xinhe Wu . Molten Intermediate Transportation-Oriented Synthesis of Amino-Rich g-C₃N₄ Nanosheets for Efficient Photocatalytic H₂O₂ Production. Acta Physico-Chimica Sinica, 2024, 40(11): 2406021-0. doi: 10.3866/PKU.WHXB202406021
15. [15]
  Jingzhao Cheng , Shiyu Gao , Bei Cheng , Kai Yang , Wang Wang , Shaowen Cao . Construction of 4-Amino-1H-imidazole-5-carbonitrile Modified Carbon Nitride-Based Donor-Acceptor Photocatalyst for Efficient Photocatalytic Hydrogen Evolution. Acta Physico-Chimica Sinica, 2024, 40(11): 2406026-0. doi: 10.3866/PKU.WHXB202406026
16. [16]
  Haodong JIN , Qingqing LIU , Chaoyang SHI , Danyang WEI , Jie YU , Xuhui XU , Mingli XU . NiCu/ZnO heterostructure photothermal electrocatalyst for efficient hydrogen evolution reaction. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1068-1082. doi: 10.11862/CJIC.20250048
17. [17]
  Haiyu Zhu , Zhuoqun Wen , Wen Xiong , Xingzhan Wei , Zhi Wang . 二维半金属/硅异质结中肖特基势垒高度的准确高效预测. Acta Physico-Chimica Sinica, 2025, 41(7): 100078-0. doi: 10.1016/j.actphy.2025.100078
18. [18]
  Peng XU , Shasha WANG , Nannan CHEN , Ao WANG , Dongmei YU . Preparation of three-layer magnetic composite Fe₃O₄@polyacrylic acid@ZiF-8 for efficient removal of malachite green in water. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 544-554. doi: 10.11862/CJIC.20230239
19. [19]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
20. [20]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036

Get Citation

PDF

XML

Metrics

PDF Downloads(19)
Abstract views(1154)
HTML views(152)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142