Citation: YANG Yang, GAO Shou-Hong, ZHANG Feng, ZHONG Ren-Qian, WANG Zhi-Peng, CHEN Wan-Sheng. Simultaneous Determination of 32 Kinds of Amino Acids in Plasma from Colorectal Cancer Patients Based on Targeted Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(7): 1083-1092. doi: 10.19756/j.issn.0253-3820.211255 shu

Simultaneous Determination of 32 Kinds of Amino Acids in Plasma from Colorectal Cancer Patients Based on Targeted Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
Department of Pharmacy, the Affiliated Huaihai Hospital of Xuzhou Medical University, the 71st Group Army Hospital of CPLA Army, Xuzhou 221004, China;
2.
Department of Pharmacy, Medical Service Center, the Affiliated Changzheng Hospital, Naval Medical University of CPLA, Shanghai 200003, China;
3.
Department of Clinical Laboratory Diagnostics, the Affiliated Changzheng Hospital, Naval Medical University of CPLA, Shanghai 200003, China

Corresponding author: WANG Zhi-Peng, CHEN Wan-Sheng,
Received Date: 27 March 2021
Revised Date: 31 March 2022

Fund Project: Supported by the International Science and Technology Cooperation and Communication Special Fund of China (No.2015DFA31810), the Scientific Research Fund of Shanghai Science and Technology Committee (No.17411972400), the Science and Technology Talent Development Project of CPLA Army, and the Xuzhou Medical Young Talent Project.

A targeted ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for simultaneous determination of 32 kinds of amino acids in plasma from colorectal cancer (CRC) patients. The chromatographic separation was performed on an Agilent Zorbax SB-C₁₈ column (150 mm×3.0 mm, 5 μm) with a binary gradient elution system (Mobile phase A:0.02% heptafluorobutyric acid and 0.2% formic acid in water; mobile phase B:methanol). The run time was 10 min. The multiple reaction monitoring mode was chosen with an electrospray ionization source operating in the positive ionization mode for data acquisition. The sample was pretreated based on protein precipitation. The average recoveries and matrix effects for 32 kinds of amino acids and 3 kinds of internal standards were 50.4%-182.4% and 41.0%-136.4%, respectively. The linear correlation coefficients were above 0.99 for 32 kinds amino acids. The intra- and inter-day accuracy for 32 kinds of amino acids ranged from -14.3% to 13.7% (RSD ≤ 8.8%) and from -14.4% to 13.4% (RSD ≤ 12.2%), respectively, and the deviations of stability under different conditions were within ±15%. This method was successfully utilized to quantify 32 kinds of amino acids in plasma samples from 6 CRC patients simultaneously.
- Amino acid,
- Colorectal cancer,
- Plasma,
- Ultra-high performance liquid chromatography-tandem mass spectrometry
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