Citation: WANG En-Peng, DU Lian-Yun, ZHU Shuang, HAN Yan-Yan, LIU Shu-Ying, CHEN Chang-Bao, LI Ping-Ya. Investigation of Pharmacokinetic Behavior of Depression Rats Caused by UVB Radiation after Oral Administration of 20(S)-Ginsenoside Rg3 by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(4): 618-627. doi: 10.19756/j.issn.0253-3820.211002 shu

Investigation of Pharmacokinetic Behavior of Depression Rats Caused by UVB Radiation after Oral Administration of 20(S)-Ginsenoside Rg3 by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China;
2.
Jilin Ginseng Academy, Changchun University of Traditional Chinese Medicine, Changchun 130117, China

Corresponding author: CHEN Chang-Bao, LI Ping-Ya,
Received Date: 2 January 2021
Revised Date: 31 January 2021

Fund Project: Supported by the Jilin Province Science and Technology Development Project(No.20200404042YY), the National key Research and Development Projects (No.2019YFC1710704) and the Jiayi Biochemical Research and Development Center (Suzhou Industrial Park) Co., Ltd.

A novel approach was developed for simultaneous determination of 20(S)-ginsenoside Rg3 (G-Rg3) and its main metabolite 20(S)-ginsenoside Rh2 (G-Rh2) in rats plasma by UHPLC-QQQ MS to compare the pharmacokinetic behavior of normal and UVB-induced depression model after oral administration of G-Rg3. In this method, an Ascentis^® Express C₁₈ chromatographic column (5 mm×3.0 mm, 2.7 μm) was used for qualitative and quantitative analysis of G-Rg3 and G-Rh2 under gradient elution with 0.1% formic acid in water-acetonitrile as the mobile phase. Simultaneously, the flow rate was 0.3 mL/min and the injection volume of sample solution was 5 μL. The MS analysis was operated in multiple-reaction monitoring (MRM) mode using electrospray ionization (ESI) under negative ion mode. With 20(S)-ginsenoside Rb1 (G-Rb1) as internal standard, G-Rg3 and G-Rh2 were quantitatively analyzed. The ions for quantification were m/z 783.5/621.4, 621.4/459 and 1107.6/954.5, respectively. The results showed that the intra-day and inter-day precisions, recovery, matrix effect and stability could meet the pharmacokinetic analysis requirement, and the whole analysis procedure could be completed in 10 minutes. Both G-Rg3 and G-Rh2 had a good linear relationship in the concentration ranges of 2.0-2500 ng/mL and 2.0-2500 ng/mL (R²>0.9907). The results indicated that the metabolic process of G-Rg3 conformed to a two-compartment pharmacokinetic model after single oral administration in the normal and model groups. The t_1/2α were (0.35±0.116) and (1.954±0.609) h, the t_1/2β were (66.103±6.425) and (52.496±33.639) h, the AUC_(0-t) were (346.75±14.108) and (551.374±117.557) mg·L/h, and the AUC_(0-t) were (497.66±39.673) and (694.523±213.077) mg·L/h. There were significant differences in pharmacokinetic parameters between the two groups (p<0.01), which was concluded that the internal environment of depression model rats might have been changed by UVB radiation, might also affecting the absorption and metabolism of G-Rg3. This method was simple, rapid, selective and sensitive, and could be used for the pharmacokinetic analysis of Rg3 and Rh2. More important, it was suitable for pharmacokinetic study of G-Rg3 in vivo.
- 20(S)-Ginsenoside Rg3,
- Rat plasma,
- Pharmacokinetics,
- Ultraviolet B radiation,
- Depression
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