Citation: LIU Xue-Tao, SUN Xi-Ru, LIU Cheng-Hui, WANG Yu-Cong. Colorimetric and Photothermal Dual-Mode Sensor for Detection of Histone[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(11): 1756-1764. doi: 10.19756/j.issn.0253-3820.210895 shu

Colorimetric and Photothermal Dual-Mode Sensor for Detection of Histone

1.
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China;
2.
School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China

Corresponding author: LIU Cheng-Hui, WANG Yu-Cong,
Received Date: 17 December 2021
Revised Date: 20 February 2022

Fund Project: Supported by the Innovation Capability Support Program of Shaanxi Province, China (No.2021TD-42) and the Program for the Fundamental Research Funds for the Central Universities of China (No.GK202101001).

Based on the peroxidase-like activity of metal-organic framework (Fe-MIL-88) and the competitive binding of histone and Fe-MIL-88 to double stranded DNA (dsDNA), a colorimetric and photothermal dual-mode sensor for detection of histone was developed by using oxidized 3,3',5,5'-tetramethylbenzidine (oxTMB) as the signal transducing probe. As a peroxidase-like nanozyme, Fe-MIL-88 could catalyze the reaction of hydrogen peroxide and 3,3',5,5'-tetramethylbenzidine (TMB) to yield oxTMB. When Fe-MIL-88 and dsDNA mixed, Fe-MIL-88 could combine with dsDNA and its peroxidase activity was blocked, resulting in the reduction of oxTMB in the reaction solution. When histone, Fe-MIL-88 and dsDNA mixed, the competitive binding of histone and Fe-MIL-88 to dsDNA could reduce the amount of dsDNA on the surface of Fe-MIL-88 and cause the recovery of Fe-MIL-88's peroxidase activity, resulting in the increase of oxTMB in the reaction solution. The production of oxTMB increased with the increase of histone concentration. OxTMB was blue with maximum absorption at 650 nm while TMB was colorless. OxTMB could also induce the increase of solution temperature under 808 nm laser irradiation because of its photothermal effect. Therefore, the quantitative determination of histone was achieved indirectly by measuring the content of oxTMB through either spectrophotometry or thermometer. The histone had a linear relationship with the absorbance of the reaction solution in the concentration range of 0.01-20.00 μg/mL using the colorimetric sensor and the detection limit of histone was estimated to be 6.7 ng/mL. Simultaneously, the histone had a linear relationship with the temperature of the reaction solution in the concentration range of 0.05-30.00 μg/mL using the photothermal sensor and the detection limit of histone was estimated to be 36 ng/mL. In addition, the method had good specificity and could be applied to detection of histone in real samples. The method was less time-consuming and the detection of histone could be completed within 60 min. The results obtained by two different sensing modes could be mutually verified to ensure the accuracy of this method. Particularly, a simple thermometer was used as the signal reader instead of large and expensive equipments in the photothermal-sensing technique. Due to the unique features of thermometer such as low cost, portability, wide accessibility and simple operation, this proposed strategy showed great potential for the point-of-care testing of histone at home and in the field in the future.
- Histone,
- Colorimetric sensing,
- Photothermal sensing,
- Metal-organic framework,
- 3,3',5,5'-Tetramethylbenzidine
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沈阳化工大学材料科学与工程学院沈阳 110142