Citation: YU Jian-Fang, SI Shi-Hui, ZHOU Zhuo, WANG Zhen-Chang, CHEN Jin-Hua. Detection of Myoglobin by High Frequency Piezoelectric Quartz Aptamer Biosensor Based on Molecular Bond Rupture Technology[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(7): 1065-1071. doi: 10.19756/j.issn.0253-3820.210881 shu

Detection of Myoglobin by High Frequency Piezoelectric Quartz Aptamer Biosensor Based on Molecular Bond Rupture Technology

1.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;
2.
State Key Laboratory of Chemical Biosensing and Metrology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

Corresponding author: SI Shi-Hui, sishihui@163.com
Received Date: 7 December 2021
Revised Date: 15 April 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.2172810).

A biosensor for detection of myoglobin based on the technology of molecular bond rupture of piezoelectric quartz crystal excited by amplitude modulation resonant and the method of double aptamer sandwich were developed. The aptamer Ⅰ was coated onto the gold electrode surface of quartz crystal microbalance (QCM) through Au-S bond, and specifically bound to myoglobin, which coupled with aptamer Ⅱ-magnetic beads to enhance the mass response. When the peak-to-peak value of the excitation voltage was increased to 8 V at the resonant frequency for 50 MHz gold-plated quartz crystal, the layer of aptamer Ⅱ-magnetic beads bonded to the surface would be removed, resulting in the occurrence of molecular bond rupture process and the increase of the resonant frequency, and the QCM aptamer biosensor with high specificity for detection of myoglobin was developed. Under the optimized conditions, the linear range of detection of myoglobin was 1.0-500 ng/mL, and the detection limit (3σ) was 0.38 ng/mL. The recoveries of myoglobin in human serum sample ranged from 96.4% to 104.0%. Compared to traditional QCM sensing technology, the sensing method established here was simple, fast, and easy to operate. Besides, the aptamer biosensor based on molecular bond rupture technology had practical and market application value.
- Quartz crystal microbalance,
- Molecular bond rupture,
- Myoglobin,
- Aptamer
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142