Citation: HU Shuang, HUA Zhen-Dong, HUANG Yu, CHENG Fang-Bin, LIU Yao. Fast Separation and Detection of Fentanyls Isomers by Ultra Performance Convergence Chromatography-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(6): 964-972. doi: 10.19756/j.issn.0253-3820.210839 shu

Fast Separation and Detection of Fentanyls Isomers by Ultra Performance Convergence Chromatography-Mass Spectrometry

HU Shuang ^1,2 ,
HUA Zhen-Dong ^3,, ,
HUANG Yu ⁴ ,
CHENG Fang-Bin ⁵ ,
LIU Yao ^1,,

1.
School of Criminal Investigation, People's Public Security University of China, Beijing 100038, China;
2.
Department of Criminal Science and Technology, Beijing Police College, Beijing 102202, China;
3.
Key Laboratory of Drug Monitoring and Control, Anti-Drug Information Technology Center of the Ministry of Public Security, Beijing 100193, China;
4.
School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China;
5.
Zhongshan Public Security Bureau Criminal Detachment, Zhongshan 528403, China

Corresponding author: HUA Zhen-Dong, LIU Yao,
Received Date: 16 November 2021
Revised Date: 5 March 2022

Fund Project: Supported by the Scientific Research Project of Beijing Municipal Education Commission(No. KM202114019001) and the Science and Technology to Strengthen Basic Work Project of Ministry of Public Security(No. 2020GABJC21).

As a new class of psychoactive substances, fentanyls have the characteristics of wide variety,rapid replacement and extremely similar structures. The accurate identification of their isomers is a major problem faced by drug analysts. Ultra performance convergence chromatography is a powerful tool to achieve efficient separation of isomers. Therefore, a new method was established for fast separation and detection of fentanyls by ultra performance convergence chromatography-mass spectrometry in this work. The separation conditions of the chromatographic column, modifiers and column temperature were optimized. The separation and detection was conducted on Waters ACQUITY UPC² BEH column with supercritical fluid CO₂ as primary mobile phase, methanol containing 20 mmol/L ammonium formate as the modifier, column temperature at 50℃ and a Xevo TQD triple quadrupole as mass spectrometer. A total of 13 kinds of fentanyls were completely separated within 6.5 min with an above 1.5-resolution to all the isomers. The chromatographic peaks were perfectly symmetric. Methodological statistics showed that, 9 kinds of common drugs did not interfere with the determination of 13 kinds of target substances;the detection limits were 0.01-0.05 ng/mL and the relative standard deviations of the retention time were less than 0.6%. Compared with the conventional ultra-high performance liquid chromatography-mass spectrometry detection method, this method had significant advantages in the separation of isomers,which fully met the anti-drug public security departments' needs for the accurate identification of fentanyls.
- Ultra performance convergence chromatography,
- Mass spectrometry,
- Fentanyls,
- Isomers,
- New psychoactive substances
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