Citation: CHEN Wei-Ni, YUAN Zi-Cheng, CAI Shen-Hui, ZOU Ying-Tong, XIN Guo-Bin, DI Dan-Dan, SONG Xin, ZHAO Peng, WU Man-Man, HU Bin. Gas Chromatography-Mass Spectrometry Analysis of Human Exhaled Volatile Organic Compounds via Wearable Facemask Microextraction Sampling[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(3): 445-453. doi: 10.19756/j.issn.0253-3820.210828 shu

Gas Chromatography-Mass Spectrometry Analysis of Human Exhaled Volatile Organic Compounds via Wearable Facemask Microextraction Sampling

CHEN Wei-Ni ¹ ,
YUAN Zi-Cheng ¹ ,
CAI Shen-Hui ¹ ,
ZOU Ying-Tong ² ,
XIN Guo-Bin ³ ,
DI Dan-Dan ¹ ,
SONG Xin ⁴ ,
ZHAO Peng ⁵ ,
WU Man-Man ^2,, ,
HU Bin ^1,,

¹Guangdong Provincial Engineering Research Center for Online Source Apportionment System of Air Pollution, Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou 510632, China;
²Guangzhou Hexin Instrument Co., Ltd, Guangzhou 510530, China;
³Key Laboratory of Forensic Toxicology, Ministry of Public Security, Beijing Municipal Public Security Bureau, Beijing 100192, China;
⁴Hefei Public Security Bureau, Hefei 230001, China;
⁵The Research Institute of Advanced Technologies, Ningbo University, Ningbo 315210, China

Corresponding author: WU Man-Man, HU Bin,
Received Date: 5 November 2021
Revised Date: 4 January 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.21804053) and the Key Laboratory of Forensic Toxicology, Ministry of Public Security, People's Republic of China (Beijing Municipal Public Security Bureau) (No.2021FTDWFX03).

Human exhaled breath contains a large number of volatile metabolites relating to human behavior and health states. In this study, solid phase microextraction (SPME) fibers were directly inserted into a facemask (SPME-in-facemask) to form a new facemask microextraction device for extracting human exhaled volatile organic compounds (VOCs). After sampling, SPME fibers were then coupled with different gas chromatography-mass spectrometers (GC-MS) for further identification of extracted VOCs, including conventional GC-MS, portable GC-MS and comprehensive two-dimensional GC-MS. Different SPME fibers and sampling time were investigated and compared. By comparing the background signals of face mask human exhaled VOCs, the effects of food intake and oral care on the compositions and changes of human exhaled VOCs were explored. The detectability and persistence of exhaled exogenous VOCs were investigated. The results showed that the facemask microextraction sampling coupled with GC-MS could be an efficient analytical tool for detecting a large amount of exhaled endogenous and exogenous VOCs. With the unique features of the facemask microextraction device, including safety, simplicity, convenient sampling, and strong adaptability to couple with different GC-MS methods in which portable GC-MS could provide a fast screening within minutes, while GC×GC-MS could give a comprehensive analysis for biomarker discovery. This work presented a novel promising strategy for investigating human health and oral diseases.
- Facemask,
- Solid-phase microextraction,
- Human exhaled breath,
- Gas chromatography-mass spectrometry,
- Volatile organic compounds
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