Citation: YANG Xu, PENG Ai-Yi, YANG Hong, XU Fei, JIN Ling-Ling. Separation of Polymethoxylated Flavonoids in Murraya Exotica L. by High Speed Countercurrent Chromatography and Analysis of Bioactivity[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(12): 2117-2125. doi: 10.19756/j.issn.0253-3820.210725 shu

Separation of Polymethoxylated Flavonoids in Murraya Exotica L. by High Speed Countercurrent Chromatography and Analysis of Bioactivity

YANG Xu ¹ ,
PENG Ai-Yi ² ,
YANG Hong ² ,
XU Fei ^1,, ,
JIN Ling-Ling ^1,,

1.
College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China;
2.
Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, School of Life Sciences, Liaoning Normal University, Dalian 116081, China

Corresponding author: XU Fei, JIN Ling-Ling,
Received Date: 3 September 2021
Revised Date: 4 November 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.81973380).

Ethanol reflux extract of Murraya exotica L was separated by macroporous resin, and 80% methanol elution component was determined to be the flavonoids-rich component as shown by the HCl-Mg reaction assay. N-Hexane-ethylacetate-methanol-water (4:6:4:6, V/V) was selected as the two-phase solvent system for high-speed countercurrent chromatography (HSCCC) based on the partition coefficients (K) and separation factors (α) values that calculated from main compounds dissolved in n-hexane, ethyl acetate, methanol and water. Purity of the isolated product was analyzed by high performance liquid chromatography. Five polymethoxylated flavonoids were isolated and determined by nuclear magnetic resonance and mass spectrometry methods. Among them, 5,7,3',4'-tetramethoxyflavone, 5,7,8,3',4',5'-hexamethoxyflavone and 5,7,3',4',5'-pentamethoxyflavone were obtained from Murraya exotica L for the first time, three compounds (5,7,3',4'-tetramethoxyflavone, 5,7,3',4',5'-pentamethoxyflavone and 5-hydroxy-6,7,3',4'-tetramethoxyflavone) potentiated cystic fibrosis transmembrane conductance regulator (CFTR) activities in dose-dependent ways. The present study established a HSCCC method for separation and analysis of low content of polymethoxylated flavonoids from Murraya exotica L., which provided foundation for the in-depth and systematic analysis of the effective substances and active chemical constituents of Murraya exotica L.
- High-speed countercurrent chromatography,
- Murraya exotica L.,
- Polymethoxylated flavonoids,
- Structural identification,
- Bioactivity
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