Citation: ZHANG Nai-Qian, FANG Qun. Progress of Single-cell Metabolite Analysis Technology Based on Microfluidic System[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(11): 1779-1791. doi: 10.19756/j.issn.0253-3820.210636 shu

Progress of Single-cell Metabolite Analysis Technology Based on Microfluidic System

ZHANG Nai-Qian ¹ ,
FANG Qun ^1,2,3,,

1.
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China;
2.
Key Laboratory of Excited-State Materials of Zhejiang Province, Zhejiang University, Hangzhou 310027, China;
3.
Hangzhou Innovation Center, Zhejiang University, Hangzhou 311200, China

Corresponding author: FANG Qun, fangqun@zju.edu.cn
Received Date: 9 July 2021
Revised Date: 30 August 2021

Fund Project: Supported by the National Major Project for Development of Scientific Instrument, China (No.21827806).

Due to the heterogeneity between different cells, the analysis of metabolites at single cell level can provide abundant information for the study of complex biological systems.Microfluidic systems can manipulate fluids in microscale structures that are close to the size of cells, making microfluidic systems suitable for single-cell analysis. In this review, current single-cell metabolite analysis technology based on microfluidic systems was introduced. Various microfluidic single-cell metabolite analysis systems based on different detection techniques including fluorescence microscopic imaging, laser induced fluorescence, electrochemistry, mass spectrometry, chemiluminescence, sensor detection technology, as well as their applications were reviewed.
- Microfluidics system,
- Single cell analysis,
- Metabolites analysis,
- Review
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