Citation: SHAO Wen-Ya, LIANG Yu, LIU Jian-Xi, LIU Hong-Tao, WANG Zhao-Wei, SUI Zhi-Gang, ZHAO Bao-Feng, ZHANG Xiao-Dan, LIANG Zhen, ZHANG Li-Hua, ZHANG Yu-Kui. O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(12): 2106-2116. doi: 10.19756/j.issn.0253-3820.210623 shu

O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice

1.
Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou 350122, China;
2.
CAS Key Lab of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
3.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
4.
College of Environmental Science and Engineering, Fujian Normal University, Fuzhou 350007, China;
5.
Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China

Corresponding author: ZHANG Li-Hua, lihuazhang@dicp.ac.cn
Received Date: 12 July 2021
Revised Date: 26 September 2021

Fund Project: Supported by the Natural Science Foundation of Fujian Province, China (No.2020J01641), the National Natural Science Foundation of China (No.21605020) and the Fund Project of Fujian Medical University (No.2018QH1007).

O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) is a single carbohydrate moiety post-translational modification that occurs on serine and threonine side chains of intracellular protein and plays an important role in nutrient metabolism. However, the great research challenges are the inherently low stoichiometry, poor ionization efficiency of O-GlcNAc peptides and no specific animo acid sequence. In this study, a strategy for quantitative detection of O-GlcNAc sites was developed by combining pseudo-isobaric dimethyl strategy, which was applied for the O-GlcNAcylation sites profiling towards the mice liver. In total, 783 O-GlcNAc sites were unambiguously quantified from high-fat fed mice liver. Among which, 122 O-GlcNAc sites were differentially expressed, corresponding to 85 O-GlcNAc proteins. Finally, the biological function of the differentially expressed O-GlcNAc proteins was analyzed.
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