Citation: GUO Jia, WEI Li-Ping, CHEN Zhi-Ying, HE Cheng-Yan. Proteome Analysis of S-Palmitoylation Modification in Clear Cell Renal Cell Carcinoma[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(9): 1563-1571. doi: 10.19756/j.issn.0253-3820.210590 shu

Proteome Analysis of S-Palmitoylation Modification in Clear Cell Renal Cell Carcinoma

1.
Department of Respiratory, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
2.
Department of Clinical Laboratory, China-Japan Union Hospital of Jilin University, Changchun 130033, China

Corresponding author: CHEN Zhi-Ying, HE Cheng-Yan,
Received Date: 26 June 2021
Revised Date: 5 August 2021

Fund Project: Supported by the Jilin Province Science and Technology Department, China (Nos.20190201215JC, 20200404193YY).

The abnormal expression of protein acyltransferase that is responsible for protein palmitoylation in renal clear cell carcinoma suggests that there may be abnormal changes of S-palmitoylation in renal clear cell carcinoma, which is closely related to metabolic disorder or activation of carcinogenic signaling pathway. In the present study, the optimized acyl-biotinyl exchange (ABE) method combined with proteomic techniques was used to analyze S-palmitoylated proteins in clear cell renal cell carcinoma. A total of 92 palmitoylated proteins were identified with high reliability, and the S-palmitoylation of adipose differentiation related protein (ADRP) was verified by Western blotting. This study provides an improved ABE method suitable for analyzing tissue samples. The identification of palmitoylated proteins in clear cell renal cell carcinoma can provide primary data for the molecular targets of early diagnosis, treatment and prognosis of clear cell renal carcinoma, which are of great significance for further study of the pathogenesis of clear cell renal carcinoma.
- S-palmitoylation,
- Clear cell renal cell carcinoma,
- Proteome,
- Acyl-biotin exchange,
- Adipose differentiation related proteins
1. [1]
  MANCINI M, RIGHETTO M, BAGGIO G. Int. J. Mol. Sci., 2020, 21(9):3378.
2. [2]
  LINEHAN W M, RICKETTS C J. Nat. Rev. Urol., 2019, 16(9):539-552.
3. [3]
  HAMMERS H. Curr. Opin. Urol., 2016, 26(6):543-547.
4. [4]
  KOTECHA R R, MOTZER R J, VOSS M H. Nat. Rev. Clin. Oncol., 2019, 16(10):621-633.
5. [5]
  JOHANN D J, WEI B R, PRIETO D A, CHAN K C, YE X, VALERA V A, SIMPSON R M, RUDNICK P A, XIAO Z, ISSAQ H J, LINEHAN W M, STEIN S E, VEENSTRA T D, BLONDER J. Anal. Chem., 2010, 82(5):1584-1588.
6. [6]
  CHEN C J, CHOU C Y, SHU K H, CHEN H C, WANG M C, CHANG C C, HSU B G, WU M S, YANG Y L, LIAO W L, YANG C, HSIAO Y T, HUANG C C. J. Proteome Res., 2021, 20(5):2953-2963.
7. [7]
  PERROUD B, ISHIMARU T, BOROWSKY A D, WEISS R H. Mol. Cell. Proteomics, 2009, 8(5):971-985.
8. [8]
  HWA J S, KIM H J, GOO B M, PARK H J, KIM C W, CHUNG K H, PARK H C, CHANG S H, KIM Y W, KIM D R, CHO G J, CHOI W S, KANG K R. Proteomics, 2006, 6(3):1077-1084.
9. [9]
  NUERRULA Y, REXIATI M, LIU Q, WANG Y J. Cancer Biomarkers, 2015, 15(4):485-491.
10. [10]
  LI H, KOSREL S A, DIMARTINO S E, HASHEMI GHEINANI A, FROEHLICH J W, LEE R S. J. Proteome Res., 2021, 20(5):2662-2672.
11. [11]
  AGGELIS V, CRAVEN R A, PENG J, HARDEN P, CAIRNS D A, MAHER E R, TONGE R, SELBY P J, BANKS R E. Proteomics, 2009, 9(8):2118-2130.
12. [12]
  PINTO J, AMARO F, LIMA A R, CARVALHO-MAIA C, JERÓNIMO C, HENRIQUE R, BASTOS M L, CARVALHO M, GUEDES DE PINHO P. Proteome Res., 2021, 20(6):3068-3077.
13. [13]
  LIN L, ZHENG J, YU Q, CHEN W, XING J, CHEN C, TIAN R. J. Proteomics, 2018, 174:9-16.
14. [14]
  ATRIH A, MUDALIAR M A, ZAKIKHANI P, LAMONT D J, HUANG JT, BRAY S E, BARTON G, FLEMING S, NABI G. Br. J. Cancer, 2014, 110(6):1622-1633.
15. [15]
16. [16]
  SUN N, WANG Y, WANG J, SUN W, YANG J, LIU N. Anal. Chem., 2020, 92(12):8292-8297.
17. [17]
18. [18]
  ZHANG J, PENG Q, ZHAO W, SUN W, YANG J, LIU N. J. Proteome Res., 2021, 20(1):110-121.
19. [19]
  CHEN J J, FAN Y, BOEHNING D. Front. Mol. Biosci., 2021, 8:656440.
20. [20]
  WANG Y, YANG W. Proteome Res., 2021, 20(1):14-26.
21. [21]
  LIU Z, LIU C, XIAO M, HAN Y, ZHANG S, XU B. Front. Oncol., 2020, 10:565414.
22. [22]
23. [23]
  SHI Q H, GAO S, SONG LN, ZHAO Y M, LI X O, WU J L, HE C Y, LI H J, ZHAO H F. Proteome Sci., 2015, 13(1):1.
24. [24]
  SUN N, SUN W, LI S, YANG J, YANG L, QUAN G, GAO X, WANG Z, CHENG X, LI Z, PENG Q, LIU N. Int. J. Mol. Sci., 2015, 16(11):25982-25998.
25. [25]
  DOWAL L, YANG W, FREEMAN M R, STEEN H, FLAUMENHAFT R. Blood, 2011, 118(13):e62-73.
26. [26]
  HU R, GUPTA R, WANG Z, WANG C, SUN H, SINGAMANENI S, KHARASCH E D, MORRISSEY J J. Kidney Int., 2019, 96(6):1417-1421.
27. [27]
  SONG Y, ZHONG L, ZHOU J, LU M, XING T, MA L, SHEN J. Proteomics Clin. Appl., 2017, 11(11-12):1700066.
28. [28]
  CAO Q, RUAN H, WANG K, SONG Z, BAO L, XU T, XIAO H, WANG C, CHENG G, TONG J, MENG X, LIU D, YANG H, CHEN K, ZHANG X. Int. J. Oncol., 2018, 53(1):137-147.
1. [1]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
2. [2]
  Yikai Wang , Xiaolin Jiang , Haoming Song , Nan Wei , Yifan Wang , Xinjun Xu , Cuihong Li , Hao Lu , Yahui Liu , Zhishan Bo . 氰基修饰的苝二酰亚胺衍生物作为膜厚不敏感型阴极界面材料用于高效有机太阳能电池. Acta Physico-Chimica Sinica, 2025, 41(3): 2406007-. doi: 10.3866/PKU.WHXB202406007
3. [3]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
4. [4]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
5. [5]
  Zhaoxin LI , Ruibo WEI , Min ZHANG , Zefeng WANG , Jing ZHENG , Jianbo LIU . Advancements in the construction of inorganic protocells and their cell mimic and bio-catalytical applications. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2286-2302. doi: 10.11862/CJIC.20240235
6. [6]
  Ze-Yuan Ma , Mei Xiao , Cheng-Kun Li , Adedamola Shoberu , Jian-Ping Zou . S-(1,3-Dioxoisoindolin-2-yl)O,O-diethyl phosphorothioate (SDDP): A practical electrophilic reagent for the phosphorothiolation of electron-rich compounds. Chinese Chemical Letters, 2024, 35(5): 109076-. doi: 10.1016/j.cclet.2023.109076
7. [7]
  Caixia Lin , Ting Liu , Zhaojiang Shi , Hong Yan , Keyin Ye , Yaofeng Yuan . Innovative Experiment of Electrochemical Dearomative Spirocyclization of N-Acyl Sulfonamides. University Chemistry, 2025, 40(4): 359-366. doi: 10.12461/PKU.DXHX202406107
8. [8]
  Dan Li , Hui Xin , Xiaofeng Yi . Comprehensive Experimental Design on Ni-based Catalyst for Biofuel Production. University Chemistry, 2024, 39(8): 204-211. doi: 10.3866/PKU.DXHX202312046
9. [9]
  Lei Shu , Zhengqing Hao , Kai Yan , Hong Wang , Lihua Zhu , Fang Chen , Nan Wang . Development of a Double-Carbon Related Experiment: Preparation, Characterization and Carbon-Capture Ability of Eggshell-Derived CaO. University Chemistry, 2024, 39(4): 149-156. doi: 10.3866/PKU.DXHX202310134
10. [10]
  Zhongyan Cao , Shengnan Jin , Yuxia Wang , Yiyi Chen , Xianqiang Kong , Yuanqing Xu . Advances in Highly Selective Reactions Involving Phenol Derivatives as Aryl Radical Precursors. University Chemistry, 2025, 40(4): 245-252. doi: 10.12461/PKU.DXHX202405186
11. [11]
  Shijie Li , Ke Rong , Xiaoqin Wang , Chuqi Shen , Fang Yang , Qinghong Zhang . Design of Carbon Quantum Dots/CdS/Ta₃N₅ S-Scheme Heterojunction Nanofibers for Efficient Photocatalytic Antibiotic Removal. Acta Physico-Chimica Sinica, 2024, 40(12): 2403005-. doi: 10.3866/PKU.WHXB202403005
12. [12]
  Jinwang Wu , Qijing Xie , Chengliang Zhang , Haifeng Shi . 自旋极化增强ZnFe_1.2Co_0.8O₄/BiVO₄ S型异质结光催化性能降解四环素. Acta Physico-Chimica Sinica, 2025, 41(5): 100050-. doi: 10.1016/j.actphy.2025.100050
13. [13]
  Zhiwen HUANG , Qi LIU , Jianping LANG . W/Cu/S cluster-based supramolecular macrocycles and their third-order nonlinear optical responses. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 79-87. doi: 10.11862/CJIC.20240184
14. [14]
  Shuyu Liu , Xiaomin Sun , Bohan Song , Gaofeng Zeng , Bingbing Du , Chongshen Guo , Cong Wang , Lei Wang . Design and Fabrication of Phospholipid-Vesicle-based Artificial Cells towards Biomedical Applications. University Chemistry, 2024, 39(11): 182-188. doi: 10.12461/PKU.DXHX202404113
15. [15]
  Kexin Dong , Chuqi Shen , Ruyu Yan , Yanping Liu , Chunqiang Zhuang , Shijie Li . Integration of Plasmonic Effect and S-Scheme Heterojunction into Ag/Ag₃PO₄/C₃N₅ Photocatalyst for Boosted Photocatalytic Levofloxacin Degradation. Acta Physico-Chimica Sinica, 2024, 40(10): 2310013-. doi: 10.3866/PKU.WHXB202310013
16. [16]
  Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
17. [17]
  Jia Huo , Jia Li , Yongjun Li , Yuzhi Wang . Ideological and Political Design of Physical Chemistry Teaching: Chemical Potential of Any Component in an Ideal-Dilute Solution. University Chemistry, 2024, 39(2): 14-20. doi: 10.3866/PKU.DXHX202307075
18. [18]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
19. [19]
  Shipeng WANG , Shangyu XIE , Luxian LIANG , Xuehong WANG , Jie WEI , Deqiang WANG . Piezoelectric effect of Mn, Bi co-doped sodium niobate for promoting cell proliferation and bacteriostasis. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1919-1931. doi: 10.11862/CJIC.20240094
20. [20]
  Peng GENG , Guangcan XIANG , Wen ZHANG , Haichuang LAN , Shuzhang XIAO . Hollow copper sulfide loaded protoporphyrin for photothermal-sonodynamic therapy of cancer cells. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1903-1910. doi: 10.11862/CJIC.20240155

Get Citation

PDF

XML

Metrics

PDF Downloads(8)
Abstract views(793)
HTML views(149)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142