Citation: SU Yong-huan, CAI Jie, YANG Ya-ni, LIU Bing-qian. A Portable Sensor Based on DNA Walker Signal Amplification for Detection of Pb²⁺[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(4): 525-534. doi: 10.19756/j.issn.0253-3820.201787 shu

A Portable Sensor Based on DNA Walker Signal Amplification for Detection of Pb²⁺

Synthetic Pharmaceutical Engineering Laboratory of Guizhou Province, School of Pharmacy, Guizhou University, Guiyang 550025, China

Corresponding author: LIU Bing-qian, nqliu@gzu.edu.cn
Received Date: 24 December 2020
Revised Date: 22 February 2022

Fund Project: Supported by the National Natural Science Foundation of China(Nos. 21864007, 21605029)the Guizhou Provincial Natural Science Foundation(Nos. Qian Ke He Ji Chu [2020]1Y042, Qian Ke He Platform for Talents [2017]5788, Qian Ke He Platform for Talents [2018]5781)

A sensitive and convenient sensor for detection of Pb²⁺ was established based on the specific recognition of Pb²⁺ and substrate DNA(sDNA), coupling with Fe₃O₄ nano-magnetic beads(MB) as sensor platform, portable glucose meter(PGM) as signal capture terminal and DNA walking device as signal amplification strategy. The nanogold-functionalized Poly(amidoamine() Au/PAMAM) dendrimers prepared by reduction method were modified by glucose oxidase(GOx) and sDNA to form sDNA/GOx/Au/PAMAM complex. Based on the complementary base pairing principle, the sDNA/GOx/Au/PAMAM complex was captured on the MB modified by capture DNA(c DNA), and the DNA enzymes(DNAzyme) could bind to the un-paired parts of sDNA. In the presence of target, Pb²⁺ could specifically recognize cleavage sites on sDNA and shear it, meanwhile released DNAzyme and GOx/Au/PAMAM. The former continued to bind with the next sDNA, and then the new identification-split-release circulation was opened.The circulation could be called DNA walker device, and the release quantity of GOx/Au/PAMAM was increased to realize signal amplification. The supernatant(GOx/Au/PAMAM), separated by magnetic separation, could oxidize glucose(Glu) in the reaction solution, and the change value of Glu concentration was monitored with PGM, thus indirectly detecting the target Pb²⁺ concentration. Under the optimal conditions, the PGM response of the system showed a good linear relationship with the logarithm of Pb²⁺ concentration ranging from 1.00 pmol/L to 1.00 μmol/L, and the detection limit was 0.66 pmol/L.
- DNA walker device,
- Signal amplification,
- Portable glucose meter,
- Lead ion(Ⅱ)
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

A Portable Sensor Based on DNA Walker Signal Amplification for Detection of Pb2+

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通讯作者: 陈斌, bchen63@163.com

A Portable Sensor Based on DNA Walker Signal Amplification for Detection of Pb²⁺