Citation: WANG Dan, QIU Wan-Ya, YU Shi-Yu, LIU Zhi-Min, ZHANG Xue-Hua, XU Zhi-Gang, ZHU Rui-Zhi, ZHANG Feng-Mei, SI Xiao-Xi, LIU Zhi-Hua. Simultaneous Analysis of Ascorbic Acid, Uric Acid and Xanthine in Human Urine by Column Tandem Technique[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(11): 1919-1925. doi: 10.19756/j.issn.0253-3820.201754 shu

Simultaneous Analysis of Ascorbic Acid, Uric Acid and Xanthine in Human Urine by Column Tandem Technique

1.
Faculty of Science, Kunming University of Science and Technology, Kunming 650500, China;
2.
R&D Center of China Tobacco Yunnan Industrial Co. Ltd., Kunming 650231, China

Corresponding author: XU Zhi-Gang, SI Xiao-Xi,
Received Date: 12 December 2020
Revised Date: 24 August 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21565018), the China National Tobacco Corporation Science and Technology Major Special Project (No.110201901002(XX-02)) and the Yunnan China Tobacco Industry Co., Ltd. Key Project (No.2018JC05).

Ascorbic acid, uric acid and xanthine in human urine are important markers of many diseases. A method for simultaneous detection of ascorbic acid, uric acid and xanthine in human urine by high performance liquid chromatography based on column tandem technique was established. The separation of these three compounds was the key to simultaneously analyze the three compounds to a certain extent because the conventional liquid chromatography could not effectively separate these highly polar substances simultaneously. The Innoval ASB C₁₈ was coupled with the hydrophilic interaction chromatography (HILIC) column to trigger off a dual-column series separation mode. The separation performance of the two columns was complementary to achieve baseline separation of the three target analytes. The results showed that a single HILIC column could not achieve the baseline separation of the three analytes. The C₁₈ column also showed very weak retention to the three analytes. The retention time was so early that the target analyte peaks might be interfered by the solvent peaks. Only the tandem of these two columns could achieve the baseline separation for the three analytes. Ultimately, an analytical method for simultaneous analysis of ascorbic acid, uric acid and xanthine in human urine with the C₁₈ column tandem HILIC column was established. The correlation coefficient was good in the linear range of 0.005-20 mg/L, and limits of detection (LODs) of ascorbic acid, uric acid, and xanthine were 1.06 μg/L, 0.94 μg/L and 0.46 μg/L, respectively. The three target analytes could be found in actual human urine by the proposed method. The contents of ascorbic acid, uric acid and xanthine were 0.47-4.70, 6.91-8.01 and 0.10-0.19 mg/L, respectively. The recoveries of the spiked urine samples were 86.2%-117.9%, and the relative standard deviation (RSD) was less than 9.3%. The established high performance liquid chromatography based on column tandem analytical method was simple, fast and efficient, and could be used for separation and simultaneous determination of ascorbic acid, uric acid and xanthine in human urine.
- Column tandem technique,
- Hydrophilic interaction chromatography column,
- C₁₈ column,
- Ascorbic acid,
- Uric acid,
- Xanthine
1. [1]
  HUSSAIN M M, ASIRI A M, RAHMAN M M. Microchem. J., 2020, 159:105534.
2. [2]
  ABELLAN-LLOBREGAT A, VIDAL L, RODRÍGUEZ-AMARO R, BERENGUER-MURCIA A, CANALS A, MORALLÓN E. Electrochim. Acta, 2017, 227:275-284.
3. [3]
  SEN S, SARKAR P. Anal. Chim. Acta, 2020, 1114:15-28.
4. [4]
  JINDAL K, TOMAR M, GUPTA V. Biosens. Bioelectron., 2014, 55(9):57-65.
5. [5]
  FEIG D I, KANG D H, JOHNSON R J. N. Engl. J. Med., 2008, 359(17):1811-1821.
6. [6]
  IBRAHIM H, TEMERK Y. J. Electroanal. Chem., 2016, 780:176-186.
7. [7]
  WANG X X, WU Q, SHAN Z, HUANG Q M. Biosens. Bioelectron., 2011, 26(8):3614-3619.
8. [8]
  WESTLEY C, XU Y, THILAGANATHAN B, CARNELL A J, TURNER N J, GOODACRE R. Anal. Chem., 2017, 89(4):2472-2477.
9. [9]
  FOWLER A A, SYED A A, KNOWLSON S, SCULTHORPE R, FARTHING D, DEWILDE C, FARTHING C A, LARUS T L, MARTIN E, BROPHY D F, GUPTA S, RESPIRATORY M, NURSING I C U, FISHER B J, NATARAJAN R. J. Transl. Med., 2014, 12(1):32.
10. [10]
11. [11]
  ZHANG F Y, WANG Z H, ZHANG Y Z, ZHENG Z X, WANG C M, DU Y L, YE W H. Talanta, 2012, 93:320-325.
12. [12]
  HASOÑ S, OSTATNÁ V, FOJTA M. Electrochim. Acta, 2020, 329:135132.
13. [13]
14. [14]
  BADOEI-DALFARD A, SOHRABI N, KARAMI Z, SARGAZI G. Biosens. Bioelectron., 2019, 141:111420.
15. [15]
  COOPER N, KHOSRAVAN R, ERDMANN C, FIENE J, LEE J W. J. Chromatogr. B, 2006, 837(1-2):1-10.
16. [16]
17. [17]
18. [18]
  SUN D, ZHANG Y, WANG F R, WU K B, CHEN J W, ZHOU Y K. Sens. Actuators, B, 2009, 141(2):641-645.
19. [19]
  ABELLÁN-LLOBREGAT A, VIDAL L, RODRÍGUEZ-AMARO R, BERENGUER-MURCIA Á, CANALS A, MORALLÓN E. Electrochim. Acta, 2017, 227:275-284.
20. [20]
  PLESKACOVA A, BREICHA S, PACAL L, KANKOVA K, TOMANDL J.Chromatographia, 2017, 80:529-536.
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
1. [1]
  Hao BAI , Weizhi JI , Jinyan CHEN , Hongji LI , Mingji LI . Preparation of Cu₂O/Cu-vertical graphene microelectrode and detection of uric acid/electroencephalogram. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1309-1319. doi: 10.11862/CJIC.20240001
2. [2]
  Jie ZHANG , Xin LIU , Zhixin LI , Yuting PEI , Yuqi YANG , Huimin LI , Zhiqiang LIU . Assembling a luminescence silencing system based on post-synthetic modification strategy: A highly sensitive and selective turn-on metal-organic framework probe for ascorbic acid detection. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 823-833. doi: 10.11862/CJIC.20230310
3. [3]
  Li'na ZHONG , Jingling CHEN , Qinghua ZHAO . Synthesis of multi-responsive carbon quantum dots from green carbon sources for detection of iron ions and L-ascorbic acid. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 709-718. doi: 10.11862/CJIC.20240280
4. [4]
  Yaping ZHANG , Tongchen WU , Yun ZHENG , Bizhou LIN . Z-scheme heterojunction β-Bi₂O₃ pillared CoAl layered double hydroxide nanohybrid: Fabrication and photocatalytic degradation property. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 531-539. doi: 10.11862/CJIC.20240256
5. [5]
  Hongyao Li , Youyan Liu , Luwei Dai , Min Yang , Qihui Wang . The Blessing of Indium Sulfide：Confronting the Narrow Path with Uric Acid. University Chemistry, 2024, 39(5): 325-335. doi: 10.3866/PKU.DXHX202311104
6. [6]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
7. [7]
  Huiying Xu , Minghui Liang , Zhi Zhou , Hui Gao , Wei Yi . Application of Quantum Chemistry Computation and Visual Analysis in Teaching of Weak Interactions. University Chemistry, 2025, 40(3): 199-205. doi: 10.12461/PKU.DXHX202407011
8. [8]
  Hailian Tang , Siyuan Chen , Qiaoyun Liu , Guoyi Bai , Botao Qiao , Fei Liu . Stabilized Rh/hydroxyapatite Catalyst for Furfuryl Alcohol Hydrogenation: Application of Oxidative Strong Metal-Support Interactions in Reducing Conditions. Acta Physico-Chimica Sinica, 2025, 41(4): 100036-. doi: 10.3866/PKU.WHXB202408004
9. [9]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
10. [10]
  Hong CAI , Jiewen WU , Jingyun LI , Lixian CHEN , Siqi XIAO , Dan LI . Synthesis of a zinc-cobalt bimetallic adenine metal-organic framework for the recognition of sulfur-containing amino acids. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 114-122. doi: 10.11862/CJIC.20240382
11. [11]
  Renxiao Liang , Zhe Zhong , Zhangling Jin , Lijuan Shi , Yixia Jia . A Palladium/Chiral Phosphoric Acid Relay Catalysis for the One-Pot Three-Step Synthesis of Chiral Tetrahydroquinoline. University Chemistry, 2024, 39(5): 209-217. doi: 10.3866/PKU.DXHX202311024
12. [12]
  Shuhui Li , Xucen Wang , Yingming Pan . Exploring the Role of Electrochemical Technologies in Everyday Life. University Chemistry, 2025, 40(3): 302-307. doi: 10.12461/PKU.DXHX202406059
13. [13]
  Kun Rong , Cuilian Wen , Jiansen Wen , Xiong Li , Qiugang Liao , Siqing Yan , Chao Xu , Xiaoliang Zhang , Baisheng Sa , Zhimei Sun . 层状MoS₂/Ti₃C₂T_x异质结光热转换材料用于太阳能驱动水蒸发. Acta Physico-Chimica Sinica, 2025, 41(6): 100053-. doi: 10.1016/j.actphy.2025.100053
14. [14]
  Ruolin CHENG , Haoran WANG , Jing REN , Yingying MA , Huagen LIANG . Efficient photocatalytic CO₂ cycloaddition over W₁₈O₄₉/NH₂-UiO-66 composite catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 523-532. doi: 10.11862/CJIC.20230349
15. [15]
  Wei Li , Guoqiang Feng , Ze Chang . Teaching Reform of X-ray Diffraction Using Synchrotron Radiation in Materials Chemistry. University Chemistry, 2024, 39(3): 29-35. doi: 10.3866/PKU.DXHX202308060
16. [16]
  Wenyan Dan , Weijie Li , Xiaogang Wang . The Technical Analysis of Visual Software ShelXle for Refinement of Small Molecular Crystal Structure. University Chemistry, 2024, 39(3): 63-69. doi: 10.3866/PKU.DXHX202302060
17. [17]
  Hao Zhao , Zhen Gao , Weihong Li . Practice and Exploration of the Construction of Experimental Technician Teams of Universities in the New Period. University Chemistry, 2024, 39(4): 7-12. doi: 10.3866/PKU.DXHX202310122
18. [18]
  Zhenjun Mao , Haorui Gu , Haiyan Che , Xufeng Lin . Exploration on Experiment Teaching of UHPLC-IC Based on Valve Switching Method. University Chemistry, 2024, 39(4): 81-86. doi: 10.3866/PKU.DXHX202311013
19. [19]
  Congying Wen , Zhengkun Du , Yukun Lu , Zongting Wang , Hua He , Limin Yang , Jingbin Zeng . Teaching Reform and Practice of Modern Analytical Technology under the Integration of Science, Industry, and Education. University Chemistry, 2024, 39(8): 104-111. doi: 10.3866/PKU.DXHX202312089
20. [20]
  Dongxue Han , Huiliang Sun , Li Niu . Virtual Reality Technology for Safe and Green University Chemistry Experimental Education. University Chemistry, 2024, 39(8): 191-196. doi: 10.3866/PKU.DXHX202312055

Get Citation

PDF

XML

Metrics

PDF Downloads(10)
Abstract views(1046)
HTML views(276)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142