Citation: REN Lin-Jiao, PENG Zheng, MENG Xiao-Long, ZHANG Pei, QIN Zi-Rui, XU Xiao-Ping, XU Peng, JIANG Li-Ying. A Split-Aptamer Sensor for Detection of Adenosine Triphosphate Based on Gold Nanoparticles[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(3): 405-412. doi: 10.19756/j.issn.0253-3820.201749 shu

A Split-Aptamer Sensor for Detection of Adenosine Triphosphate Based on Gold Nanoparticles

College of Electrical and Information, Zhengzhou University of Light Industry Engineering, Zhengzhou 450000, China

Corresponding author: ZHANG Pei, JIANG Li-Ying,
Received Date: 11 December 2020
Revised Date: 6 January 2022

Fund Project: Supported by the National Natural Science Foundation of China (Nos.62073299, 61801436), the Henan University Science and Technology Innovation Team Project (No.20IRTSTHN017), the Henan Province Key Research and Development and Promotion Projects (No.202102210186) and the Key Scientific Research Projects of Colleges and Universities in Henan Province, China (No.21A410001).

A split aptamer sensor based on gold nanoparticles (AuNPs) was constructed for detection of adenosine triphosphate (ATP). The ATP aptamer was splited into two fragments (P1 and P2), of which the 5' end of P1 fragment was modified by carboxyfluorescein (FAM) fluorophore, while the P2 fragment with 3' end thiol-functionalization was modified on the AuNPs surface through the self-assembly method by Au-S bond. It was found that the P1 and P2 fragments could not combine together before adding ATP, and the distance between P1 fragments and AuNPs was relatively far, which resulted in strong fluorescence signal. However, after adding ATP into the sensing system, the sandwich structure of P1-ATP-P2 formed, and the FAM fluorophore in the 5' end of P1 fragments approached to the AuNPs, leading to the decrease of the fluorescence intensity due to fluorescence resonance energy transfer effect. Under optimal experimental conditions, the fluorescence intensity of the sensor showed linear relationship with ATP concentration in the range of 0.03-3.33 nmol/L and 3.33-15 nmol/L, respectively, with a detection limit (S/N=3) of 0.03 nmol/L. Furthermore, the constructed sensor had excellent specificity for detection of ATP.
- Split aptamers,
- Fluorescence sensor,
- Gold nanoparticles,
- Fluorescence resonance energy transfer,
- Adenosine triphosphate
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