Citation: PANG Bo, MENG Xin, BIAN Xin-Yu, LIU Shu, PI Zi-Feng, LIU Zhi-Qiang, SONG Feng-Rui. Interaction Studies of Human Islet Amyloid Polypeptide and Flavonols by Electrospray Ionization-Ion Mobility-Mass Spectrometry and Fluorescence Spectroscopy[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(11): 1881-1889. doi: 10.19756/j.issn.0253-3820.201729 shu

Interaction Studies of Human Islet Amyloid Polypeptide and Flavonols by Electrospray Ionization-Ion Mobility-Mass Spectrometry and Fluorescence Spectroscopy

PANG Bo ^1,2 ,
MENG Xin ^1,2 ,
BIAN Xin-Yu ^1,2 ,
LIU Shu ¹ ,
PI Zi-Feng ¹ ,
LIU Zhi-Qiang ¹ ,
SONG Feng-Rui ^1,,

1.
Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230029, China

Corresponding author: SONG Feng-Rui, songfr@ciac.ac.cn
Received Date: 4 December 2020
Revised Date: 20 September 2021

Fund Project: Supported by the National Natural Science Foundation of China (Nos.21673219, 81873193).

The interaction of human islet amyloid polypeptide (hIAPP) with five kinds of flavonols (Astragalin, isoquercitrin, isorhamnetin-3-O-neohesperidoside, isorhamnetin-3-O-rutinoside, rutin) was investigated by electrospray ionization-ion mobility-mass spectrometry (ESI-IM-MS) and fluorescence spectroscopy. The results showed that the glycosyl groups and hydroxyl groups of aglycones in flavonols could improve their binding affinity with hIAPP, and enhance the gas phase stability of the complexes formed by hIAPP and ligands. However, the effects of multiple hydroxyl groups of aglycones on IAPP aggregation was more obvious, followed by the number of glycosyl groups and different types of glycosyl groups. Among the five flavonols, the complexes formed by rutin and hIAPP were the most stable and rutin had the strong inhibitory action on the fibrillation of hIAPP. IM-MS analysis results showed that rutin could stabilize the conformation of hIAPP by binding to hIAPP. The obtained results were useful to understand the structure-activity of the interaction of flavonols with hIAPP and provide valuable reference for the screening of potent amyloid inhibitors from natural products.
- Human islet amyloid polypeptide,
- Flavonols,
- Electrospray ionization-ion mobility-mass spectrometry,
- Fluorescence spectroscopy,
- Interaction
1. [1]
  MUKHERJEE A, MORALES-SCHEIHING D, BUTLER P C, SOTO C. Trends Mol. Med., 2015, 21(7):439-449.
2. [2]
  HU F, QIU X, BU S. Arch. Physiol. Biochem., 2020, 126(3):235-241.
3. [3]
  COSTES S. Curr. Opin. Pharmacol., 2018, 43(1):104-110.
4. [4]
  KIRIYAMA Y, NOCHI H. Cells, 2018, 7(8):1-13.
5. [5]
  TOMASELLO M F, SINOPOLI A, PAPPALARDO G. J. Diabetes Res., 2015, 2015:918573.
6. [6]
  WESTERMARK P, ANDERSSON A, WESTERMARK G T. Physiol. Rev., 2011, 91(3):795-826.
7. [7]
  ASTHANA S, MALLICK B, ALEXANDRESCU A T, JHA S. Biochim. Biophys. Acta, Biomembr., 2018, 1860(9):1765-1782.
8. [8]
  SEQUEIRA I R, POPPITT S D. Nutrients, 2017, 9(7):788.
9. [9]
  ALAM P, SIDDIQI K, CHTURVEDI S K, KHAN R H. Int. J. Biol. Macromol., 2017, 103(1):208-219.
10. [10]
  GLEVITZKY I, DUMITREL G A, GLEVITZKY M, PASCA M B, MARIAP. Rev. Chim. (Bucharest, Rom.), 2019, 70(9):3103-3107.
11. [11]
  BALDIM J L, DE ALCANTARA B G V, DOMINGOS O D S, SOARES M G, CALDAS I S, NOVAES R D, OLIVEIRA T B, LAGO J H G, CHAGAS-PAULA D A. Oxid. Med. Cell. Longevity, 2017, 2017:3789856.
12. [12]
  WANG L, SONG J, LIU A, XIAO B, LI S, WEN Z, LU Y, DU G. Nat. Prod. Bioprospect., 2020, 10(5):271-283.
13. [13]
  CIUMARNEAN L, MILACIU M V, RUNCAN O, VESA S C, RACHISAN A L, NEGREAN V, PERNE M G, DONCA V I, ALEXESCU T G, PARA I, DOGARU G. Molecules, 2020, 25(18):4320.
14. [14]
  RAFFA D, MAGGIO B, RAIMONDI M V, PLESCIA F, DAIDONE G. Eur. J. Med. Chem., 2017, 142:213-228.
15. [15]
16. [16]
  XU L, LI Y, DAI Y, PENG J. Pharmacol. Res., 2018, 130(1):451-465.
17. [17]
  PITHADIA A, BRENDER J R, FIERKE C A, RAMAMOORTHY A. J. Diabetes Res., 2016, 2016:2046327.
18. [18]
  CARMONA V, MARTIN-ARAGON S, GOLDBERG J, SCHUBERT D, BERMEJO-BESCOS P. Nutr. Neurosci., 2020, 23(8):575-590.
19. [19]
  HE J, WANG Y, CHANG A K, XU L, WANG N, CHONG X, LI H, ZHANG B, JONES G W, SONG Y. J. Agric. Food Chem., 2014, 62(39):9442-9449.
20. [20]
  CHEN S, LIU Y,ZHOU Y, HE L, OUYANG J. Analyst, 2020, 145(22):7289-7296.
21. [21]
  YU X L, LI Y N, ZHANG H, SU Y J, ZHOU W W, ZHANG Z P, WANG S W, XU P X, WANG Y J, LIU R T. Food Funct., 2015, 6(10):3296-3306.
22. [22]
  KIM J H, LEE S, CHO E J. Nutrients, 2020, 12(1353):1-15.
23. [23]
  TROTT O, OLSON A J. J. Comput. Chem., 2010, 31(2):455-461.
24. [24]
  MORRIS G M, HUEY R, LINDSTROM W, SANNER M F, BELEW R K, GOODSELL D S, OLSON A J. J. Comput. Chem., 2009, 30(16):2785-2791.
25. [25]
  DARMANIN C, CHEVREUX G, POTIER N, DORSSELAER A V, HAZEMANN I, PODJARNY A, EL-KABBANI O. Bioorg. Med. Chem., 2004, 12(14):3797-3806.
26. [26]
  ZHAO B, ZHUANG X, PI Z, LIU S, LIU Z, SONG F. J. Am. Soc. Mass Spectrom., 2018, 29(4):734-741.
27. [27]
  EL-KABBANI O, ROGNIAUX H, BARTH P, CHUNG R P T, FLETCHER E V, DORSSELAER A V, PODJARNY A. Proteins, 2000, 41(3):407-414.
28. [28]
  JIA L, WANG Y, SANG J, CUI W, ZHAO W, WEI W, CHEN B, LU F, LIU F. J. Agric. Food Chem., 2019, 67(14):3946-3955.
29. [29]
  ROVNYAGINA N R, SLUCHANKO N N, TIKHONOVA T N, FADEEV V V, LITSKEVICH A Y, MASKEVICH A A, SHIRSHIN E A. Int. J. Biol. Macromol., 2018, 108(1):284-290.
30. [30]
  XUE C, LIN T Y, CHANG D, GUO Z. R. Soc. Open Sci., 2017, 4(1):160696.
31. [31]
  KAYED R, BERNHAGEN J, GREENFIELD N, SWEIMEH K, BRUNNER H, VOELTER W, KAPURNIOTU A. J. Mol. Biol., 1999, 287(4):781-796.
32. [32]
  DUPUIS N F, WU C, SHEA J E, BOWERS M T. J. Am. Chem. Soc., 2009, 131(51):18283-18292.
33. [33]
  DUPUIS N F, WU C, SHEA J E, BOWERS M. J. Am. Chem. Soc, 2011, 133(19):7240-7243.
1. [1]
  Yahui HAN , Jinjin ZHAO , Ning REN , Jianjun ZHANG . Synthesis, crystal structure, thermal decomposition mechanism, and fluorescence properties of benzoic acid and 4-hydroxy-2, 2′: 6′, 2″-terpyridine lanthanide complexes. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 969-982. doi: 10.11862/CJIC.20240395
2. [2]
  Chuanming GUO , Kaiyang ZHANG , Yun WU , Rui YAO , Qiang ZHAO , Jinping LI , Guang LIU . Performance of MnO₂-0.39IrO_x composite oxides for water oxidation reaction in acidic media. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1135-1142. doi: 10.11862/CJIC.20230459
3. [3]
  Hailian Tang , Siyuan Chen , Qiaoyun Liu , Guoyi Bai , Botao Qiao , Fei Liu . Stabilized Rh/hydroxyapatite Catalyst for Furfuryl Alcohol Hydrogenation: Application of Oxidative Strong Metal-Support Interactions in Reducing Conditions. Acta Physico-Chimica Sinica, 2025, 41(4): 100036-. doi: 10.3866/PKU.WHXB202408004
4. [4]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
5. [5]
  Huiying Xu , Minghui Liang , Zhi Zhou , Hui Gao , Wei Yi . Application of Quantum Chemistry Computation and Visual Analysis in Teaching of Weak Interactions. University Chemistry, 2025, 40(3): 199-205. doi: 10.12461/PKU.DXHX202407011
6. [6]
  Chun-Lin Sun , Yaole Jiang , Yu Chen , Rongjing Guo , Yongwen Shen , Xinping Hui , Baoxin Zhang , Xiaobo Pan . Construction, Performance Testing, and Practical Applications of a Home-Made Open Fluorescence Spectrometer. University Chemistry, 2024, 39(5): 287-295. doi: 10.3866/PKU.DXHX202311096
7. [7]
  Zhaoxuan ZHU , Lixin WANG , Xiaoning TANG , Long LI , Yan SHI , Jiaojing SHAO . Application of poly(vinyl alcohol) conductive hydrogel electrolytes in zinc ion batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 893-902. doi: 10.11862/CJIC.20240368
8. [8]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
9. [9]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
10. [10]
  Yingran Liang , Fei Wang , Jiabao Sun , Hongtao Zheng , Zhenli Zhu . Construction and Application of a New Experimental Device for Determination of Alkaline Metal Elements by Plasma Atomic Emission Spectrometry Based on Solution Cathode Glow Discharge: An Alternative Approach for Fundamental Teaching Experiments in Emission Spectroscopy. University Chemistry, 2024, 39(5): 380-387. doi: 10.3866/PKU.DXHX202312024
11. [11]
  Yiling Wu , Peiyao Jin , Shenyue Tian , Ji Zhang . The Star of Sugar Substitutes: An Interview of Erythritol. University Chemistry, 2024, 39(9): 22-27. doi: 10.12461/PKU.DXHX202404034
12. [12]
  Zongyuan Chen , ChunSheng Shi , Yiwen Li , Ganlin Zu , Qiang Jin , Haishan Wang , Fujun Wang , Dekun Yan , Zhijun Guo , Wangsuo Wu . Measurement of Uranium Isotopes in Environmental Water Samples by Alpha-Spectroscopy: Design of an Undergraduate Radiochemistry Experiment. University Chemistry, 2025, 40(4): 353-358. doi: 10.12461/PKU.DXHX202406103
13. [13]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
14. [14]
  Aili Feng , Xin Lu , Peng Liu , Dongju Zhang . Computational Chemistry Study of Acid-Catalyzed Esterification Reactions between Carboxylic Acids and Alcohols. University Chemistry, 2025, 40(3): 92-99. doi: 10.12461/PKU.DXHX202405072
15. [15]
  Wenliang Wang , Weina Wang , Lixia Feng , Nan Wei , Sufan Wang , Tian Sheng , Tao Zhou . Proof and Interpretation of Severe Spectroscopic Selection Rules. University Chemistry, 2025, 40(3): 415-424. doi: 10.12461/PKU.DXHX202408063
16. [16]
  Yanglin Jiang , Mingqing Chen , Min Liang , Yige Yao , Yan Zhang , Peng Wang , Jianping Zhang . Experimental and Theoretical Investigations of Solvent Polarity Effect on ESIPT Mechanism in 4′-N,N-diethylamino-3-hydroxybenzoflavone. Acta Physico-Chimica Sinica, 2025, 41(2): 100012-. doi: 10.3866/PKU.WHXB202309027
17. [17]
  Yan ZHAO , Xiaokang JIANG , Zhonghui LI , Jiaxu WANG , Hengwei ZHOU , Hai GUO . Preparation and fluorescence properties of Eu³⁺-doped CaLaGaO₄ red-emitting phosphors. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1861-1868. doi: 10.11862/CJIC.20240242
18. [18]
  Linhan Tian , Changsheng Lu . Discussion on Sextuple Bonding in Diatomic Motifs of Chromium Family Elements. University Chemistry, 2024, 39(8): 395-402. doi: 10.3866/PKU.DXHX202401056
19. [19]
  Yanan Jiang , Yuchen Ma . Brief Discussion on the Electronic Exchange Interaction in Quantum Chemistry Computations. University Chemistry, 2025, 40(3): 10-15. doi: 10.12461/PKU.DXHX202402058
20. [20]
  Zhanggui DUAN , Yi PEI , Shanshan ZHENG , Zhaoyang WANG , Yongguang WANG , Junjie WANG , Yang HU , Chunxin LÜ , Wei ZHONG . Preparation of UiO-66-NH₂ supported copper catalyst and its catalytic activity on alcohol oxidation. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 496-506. doi: 10.11862/CJIC.20230317

Get Citation

PDF

XML

Metrics

PDF Downloads(9)
Abstract views(706)
HTML views(146)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142