Citation: YANG Cheng, ZHANG Ya-Qi, LIN Dai-Qin, LIU Yang, SUN Bing-Bing. Chemiluminescence Sensor Based on Composite Functional Nucleic Acid for Detection of Ochratoxin A in Wine[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(4): 496-503. doi: 10.19756/j.issn.0253-3820.201705 shu

Chemiluminescence Sensor Based on Composite Functional Nucleic Acid for Detection of Ochratoxin A in Wine

1.
State Key Laboratory of Fine Chemicals, School of Chemistry, College of Chemical Engineering, Dalian University of Technology, Dalian 116024, China;
2.
Jiangxi Product Quality Supervision Testing Institute, Nanchang 330052, China;
3.
Key Laboratory of Life Organophosphorus Chemistry and Chemical Biology of Ministry of Education, Tsinghua University, Beijing 100084, China

Corresponding author: YANG Cheng, LIN Dai-Qin,
Received Date: 25 November 2020
Revised Date: 14 December 2020

Fund Project: Supported by the National Natural Science Foundation of China (No. 201874080), the Fundamental Research Funds for the Central Universities (No. DUT17LAB18, DUT15RC(3)064), the Open Funds of the State Key Laboratory of Electroanalytical Chemistry (No. SKLEAC202105), and the Open Funds of the Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, China.

A chemiluminescence sensor, using ochratoxin A (OTA) aptamer as the activation switch of the split DNAzyme, is established to achieve high-sensitivity detection of OTA in wine. Guanine-rich oligonucleotide can fold into a parallel G-quadruplex in the presence of K⁺. Hemin as a ligand is able to specifically bind to parallel G-quadruplexes. Hemin-G-quadruplex complexes display peroxidase-like activity, named as DNAzyme. The activity of the DNAzyme can be measured by chemiluminescence via the H₂O₂ mediated-oxidation with luminol. Guanine-rich oligonucleotide splits into two fragments, and when they are near to each other, they can reform DNAzyme. OTA aptamer as a switch is inserted into the nucleic acid sequence of DNAzyme. In the absence of OTA, the unfolded OTA aptamer inhibits the split DNAzyme effectively self-assemble to form a structure with enzymatic activity. In the presence of OTA, OTA induces the aptamer to form an antiparallel G-quadruplex and draws the tail of the aptamer closer, so that the two fragments of split DNAzyme refold and complexes with hemin. Folding degree of the aptamer will directly affect the activity of DNAzyme. The high-sensitive detection of OTA can be obtained through monitoring the enhancing ratio of chemiluminescence intensity. The linear range of this method is 0.10-2.00 nmol/L, and the limit of detection is 0.10 nmol/L.
- Functional nucleic acid,
- Aptamer,
- Split DNAzyme,
- Chemiluminescence,
- Ochratoxin A
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沈阳化工大学材料科学与工程学院沈阳 110142