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Citation: YANG Hai-Zhu, CHU Ya-Ru, WANG Qing-Xiang, DING Cai-Feng, GAO Feng. An Electrochemical Biosensor for Cardiac Troponin I Based on Electrocatalytic Activity Suppression of MIL-101(Fe) by Immunoreaction[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(5): 789-797. doi: 10.19756/j.issn.0253-3820.201693 shu

An Electrochemical Biosensor for Cardiac Troponin I Based on Electrocatalytic Activity Suppression of MIL-101(Fe) by Immunoreaction

1.
Department of Chemistry and Environment Science, Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou 363000, China;
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Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China

Corresponding author: GAO Feng, Fgao1981@126.com
Received Date: 20 November 2020
Revised Date: 27 January 2021

Fund Project: Supported by the National Natural Science Foundation of China (Nos.21802064, 21275127), the Natural Science Foundation of Fujian Province, China (Nos.2019J05108, 2018J01435), and the Foundation of Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Qingdao University of Science and Technology.

The metal-organic framework material of MIL-101(Fe) with H₂O₂ mimicking enzyme activity was synthesized by a typical hydrothermal method. MIL-101(Fe) and cardiac troponin I antibody (anti-cTnI) were then successively immobilized on the surface of glassy carbon electrode that was pre-modified with amino-modified graphene (NH₂-GR) through the covalent bonding. Thus, a novel MIL-101(Fe)/NH₂-GR-based electrochemical sensing interface for cTnI was constructed. Electrochemical experiments showed that the immunoreaction between anti-cTnI and target cTnI on the sensing interface significantly suppressed the electrocatalytic activity of electrode-confined MIL-101(Fe) to H₂O₂, realizing the label-free analysis of cTnI. Under the optimal conditions, the catalytic current difference (ΔI) and the negative logarithm of the cTnI concentration (-lgC_cTnI) showed a good linear relationship in the concentration range of 10 fg/mL-0.1 μg/mL, and the detection limit was 3.1 fg/mL (S/N=3). The constructed sensor was applicable for detection of cTnI in actual human serum samples, and the recoveries were 96.0%-103.0%. The sensor exhibited good selectivity and reproducibility.
- MIL-101(Fe),
- Hydrogen peroxide mimic enzyme,
- Chronoamperometry,
- Cardiac troponin I,
- Immune reaction
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