Citation: SHI Rui, QIN Xiu, YUAN Chun-Ling, CHENG Shi-Qi, WANG Yi-Lin. Colorimetric Determination of Uric Acid with Molybdenum Disulfide Nanosheets as Peroxidase Mimetic Enzyme[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(3): 397-406. doi: 10.19756/j.issn.0253-3820.201644 shu

Colorimetric Determination of Uric Acid with Molybdenum Disulfide Nanosheets as Peroxidase Mimetic Enzyme

School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China

Corresponding author: WANG Yi-Lin, theanalyst@163.com
Received Date: 2 November 2020
Revised Date: 24 December 2020

Fund Project: National Natural Science Foundation of China (No. 61664002) and the Innovation Project of Guangxi Graduate Education (No. YCSW2020016).

MoS₂ nanosheets with peroxidase-like activity were prepared by one-step hydrothermal method. The obtained MoS₂ nanosheets could catalyze H₂O₂-mediated oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB) to produce corresponding blue oxidized product (ox-TMB). Thus, a simple method for detection of H₂O₂ was established. Under the optimized conditions such as pH 3.7, temperature of 35℃, 1.0 μg/mL MoS₂ and incubation time of 40 min, the absorbance of ox-TMB increased with the increase of H₂O₂ concentration. Given the fact that H₂O₂ was one of the products of uric acid oxidase-catalyzed uric acid oxidation, a method for the indirect detection of uric acid based on MoS₂ nanosheets-catalyzed and H₂O₂-mediated TMB oxidation was developed. The increase of ox-TMB absorbance enabled the sensitive determination of uric acid in the lineatrange of 2.5-40 μmol/L and 40-100 μmol/L, respectively, and the detection limit was 0.093 μmol/L (3σ/k). The method exhibited potential application in the detection of uric acid in urine and serum samples with recoveries of 92.6%-102.0%.
- Molybdenumdisulfide,
- Nanosheets,
- Mimetic enzyme,
- Detection,
- Uric acid
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