Citation: GUO Zhen, ZHOU Xia, ZHAO Bo, ZHAO Guang-Liang, LI Xian-Jiang, LI Xiu-Qin, ZHANG Qing-He. Determination of New Illegal Additives in Wheat Flour and Flour Treatment Agent by High Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(4): 504-511. doi: 10.19756/j.issn.0253-3820.201632 shu

Determination of New Illegal Additives in Wheat Flour and Flour Treatment Agent by High Performance Liquid Chromatography

Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China

Corresponding author: LI Xiu-Qin, lixq@nim.ac.cn
Received Date: 29 October 2020
Revised Date: 19 February 2021

Fund Project: Supported by the Special Project of State Administration for Market Regulation for Technical Support (No.2020YJ040).

A method for determination of four kinds of illegal additives kojic acid, benzodiazepine acid, 2-mercapto-5-methyl-1,3,4-thiadiazole and thiabendazole in wheat flour and flour treatment agent was developed by using high performance liquid chromatography (HPLC). The sample was first extracted with methanol. Then the analytes were separated on a ZORBAX Eclipse Plus C₁₈ column, separated by gradient elution with methanol and 0.01% H₃PO₄ aqueous solution, detected by diode array detector, and quantified by external standard method. The chromatographic conditions and pretreatment methods were optimized. Effects of several important factors, including influence of mobile phase composition on separation degree and the improvement of extraction efficiency by different extraction solvents, were investigated to acquire optimum conditions. The results showed that the 4 kinds of illegal additives had good linear relationship in the concentration range of 0.1-2.0 μg/mL (R² ≥ 0.9998). The limits of detection (LODs, S/N ≥ 3) for the 4 kind of illegal additives were 0.3-0.7 mg/kg, and the recoveries in wheat flour and flour treatment agent were 80.8%-95.9% at 3 spiking levels with the relative standard deviations (RSDs) of 0.5%-4.9% (n=6). This method was simple, accurate, and had good purification effect, which was suitable for the screening and determination of illegal additives in wheat flour and flour treatment agent.
- Wheat flour,
- Flour treatment agent,
- High performance liquid chromatography,
- Kojic acid,
- Benzohydroxamic acid,
- 2-Mercapto-5-methyl-1,3,4-thiadiazole,
- Thiabendazole
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
1. [1]
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