Citation: MA Dan-Ying, FU Juan-Juan, TANG Jin-Lan, BAO Yi-Lin, SHANG Lin-Wei, WANG Xiao, YIN Jian-Hua. Study on Changes of Breast Cancer Tissue Composition by Raman Spectroscopy[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(1): 144-150. doi: 10.19756/j.issn.0253-3820.201537 shu

Study on Changes of Breast Cancer Tissue Composition by Raman Spectroscopy

Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China

Corresponding author: YIN Jian-Hua, yin@nuaa.edu.cn
Received Date: 3 September 2020
Revised Date: 11 November 2020

Fund Project: Supported by the National Natural Science Foundation of China (No.61378087) and the Six Talent Peaks Project of Jiangsu Province, China(No.SWYY-034).

Raman spectroscopic technique is powerful to obtain microscopic information of tissue lesions at molecular level. In this study, Raman microspectroscopy system was used to collect in situ Raman spectra of the healthy breast and breast cancer tissues of 20 patients to investigate the biochemical composition changes. By comparing the average Raman spectra of healthy and cancerous breast tissues, it was clearly shown that the spectral intensity of cancerous tissues was significantly lower than that of healthy breast tissues. As comparing Raman intensity ratios of the protein and lipid components between the two types of tissues, it was found that the lipid content decreased and the protein content increased in cancerous tissues. In the meantime, saturated fatty acids were converted into unsaturated fatty acids in the process of tissue canceration. And it was further found that polyunsaturated fatty acids increased in content and monounsaturated fatty acids decreased in content after tissue canceration. This study revealed many biochemical component changes in the canceration process of breast tissue, especially the changes in fatty acid content, which was of great significance for the study of the biochemical mechanism of breast cancer, and was helpful for early monitoring and spectroscopic diagnosis of breast cancer.
- Raman spectroscopy,
- Breast cancer,
- Lipid,
- Protein,
- Fatty acid
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