Citation: WEI Xing, ZHOU Jing-Jing, LIU Jin-Hui, CHEN Ming-Li, WANG Jian-Hua. Determination of Chromium Based on Laser Ablation Inductively Coupled Plasma Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(3): 432-439. doi: 10.19756/j.issn.0253-3820.201514 shu

Determination of Chromium Based on Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Department of Chemistry, College of Science, Northeastern University, Shenyang 110819, China

Corresponding author: CHEN Ming-Li, WANG Jian-Hua,
Received Date: 24 August 2020
Revised Date: 22 November 2020

Fund Project: National Natural Science Foundation of China (Nos. 21727811, 21675019

Chromium plays an irreplaceable role in the life system. The exploration of chromium is beneficial to reveal its physiological or pathological processes. In this study, a new method was established for determination of chromium in gels by matrix-matched external standard method-laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The relative standard deviation of chromium signal intensity during ablation standard was less than 16% (n=300), the correlation coefficient (R²) of LA-ICP-MS quantitative calibration curve was 0.9805, and the detection limit of method was 2.22 μg/g. MCF-7 cells were used as an in vitro cell model, and two kinds of polyacrylamide gel electrophoresis (PAGE) methods were used to separate the protein extract after chromium incubation. And then, the electrophoresis bands obtained by two electrophoresis methods were ablated respectively by LA-ICP-MS with line scan mode, and the weak chromium-containing protein bands were obtained in electrophoresis band. Finally, the LA-ICP-MS was used to measure and image the chromium in individual cell, and it was found that cell element distribution map overlapped well with the cell contour map under the laser microscope.
- Matrix-matched external standard method,
- Chromium,
- Laser ablation inductively coupled plasma mass spectrometry,
- Gel electrophoresis
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