Citation: XU Xia, LI Ting, CAO Li-Bo, JIA Jin-Ru, GONG Xing-Cheng, LI Jun, TU Peng-Fei, WANG Yi-Tao, SONG Yue-Lin. Chemical Profiling of Peucedani Radix by Achiral-chiral Liquid Chromatography-Predictive Multiple Reaction Monitoring[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(12): 1717-1727. doi: 10.19756/j.issn.0253-3820.201485 shu

Chemical Profiling of Peucedani Radix by Achiral-chiral Liquid Chromatography-Predictive Multiple Reaction Monitoring

XU Xia ¹ ,
LI Ting ¹ ,
CAO Li-Bo ¹ ,
JIA Jin-Ru ¹ ,
GONG Xing-Cheng ¹ ,
LI Jun ^1,2 ,
TU Peng-Fei ¹ ,
WANG Yi-Tao ³ ,
SONG Yue-Lin ^1,2,,

1.
Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China;
2.
Beijing Key Lab for Quality Evaluation of Chinese Meteria Medica, Beijing University of Chinese Medicine, Beijing 100029, China;
3.
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa 999078, Macao, China

Corresponding author: SONG Yue-Lin, syltwc2005@163.com
Received Date: 11 August 2020
Revised Date: 14 September 2020

Fund Project: This work was supported by the National Key Research and Development Project of China (No. 2018YFC1707300), the National Natural Science Foundation of China (Nos. 81773875, 81530097), the Young Elite Scientists Sponsorship Program by CAST (No. 2017QNRC001), the Young Scientist Program by Beijing University of Chinese Medicine (No. BUCM-2019-QNKXJB006), and the Fundamental Research Funds for the Central Universities, China (No. 2020-JYB-ZDGG-041).

Peucedani radix and other herbal medicines are not only complex in chemical composition, but also often contain a large number of enantiomers, and there are often differences in content of enantiomers. In this work, a new analytical platform namely achiral-chiral liquid chromatography (LC)-predictive multiple reaction monitoring was firstly configured for chemo- and enantio-selective separation of the components in Peucedani Radix, a well-known herbal medicine. Serially coupled achiral LC and chiral LC integrated the robust separation potential of RP-C₁₈ column along with the enantio-separation advantage from chiral column, thus addressing the disadvantages of heart-cutting achiral-chiral two dimensional LC in terms of sophisticated instrumentation and unsatisfactory precision. The narrow-bore core-shell RP-C₁₈ column was served as the front tool to accomplish efficient chemo-selective separation within those coumarins bearing similar chemical structures, a dilution pump was introduced between the two columns to facilitate the optimization of optimum elution program from the chiral column, and a wide-bore chiral column offered the enantio-separation potential for those enantiomers. Predictive MRM mode was deployed to sensitively detect the chemical components, and the enhanced product ion program was utilized to acquire MS² spectra for all signals. Structure confirmation was conducted by matching the fragment ion species with the well-proposed fragmentation rules. As a result, 61 components, in total, were found from Peucedani Radix, including eleven pairs of enantiomers. Above all, achiral-chiral LC-predictive MRM is a promising tool for the quantitative and qualitative analysis of the complicated matrices, beyond herbal medicines, which are featured by enantiomerically enriched mixtures.
- Achiral-chiral liquid chromatography,
- Predictive multiple reaction monitoring,
- Enantioseparation,
- Angular-type pyranocoumarins,
- Chinese medicine,
- Peucedani radix
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