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Citation: ZHU Liu, ZHANG Nai-Qian, FANG Pan, PAN Jian-Zhang, WONG Catherine Chiu-Lan, FANG Qun. Development and Application of Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry System Based on A Coaxial Sheath Flow Interface[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(1): 34-41. doi: 10.19756/j.issn.0253-3820.201408 shu

Development and Application of Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry System Based on A Coaxial Sheath Flow Interface

1.
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China;
2.
Center for Precision Medicine Multi-omics Research, Peking University Health Science Center, Beijing 100191, China

Corresponding author: WONG Catherine Chiu-Lan, FANG Qun,
Received Date: 13 July 2020
Revised Date: 31 August 2020

Fund Project: Supported by the National Natural Science Foundation of China (Nos. 21827806, 21435004).

A coaxial sheath-flow capillary electrophoresis-electrospray ionization mass spectrometry (CE-ESI-MS) interface based on dual capillaries was developed for performing rapid, efficient and sensitive analysis. By designing different electrode systems, the online combination between homemade interface and different ESI-MS systems was achieved. The CE-ESI-MS system utilized the suction effect to accomplish hydrodynamic injection without external force, and realized the introduction of picoliter samples. The interface had many advantages such as simple to build, low cost, good durability, minimal dead volume and low dilution effect based on optimized parameters. The spray stability was evaluated by continuous injection with a relative standard deviation (RSD) of 3.4%. Therefore, in the nine consecutive separations of reserpine, the RSD of retention time, full Width at Half Maximum and peak area were 1.9%, 9.2% and 8.2%, respectively. The present system was successfully applied to separate eighteen standard amino acids within 9 min. Moreover, the baseline separation of five standard peptides mixture was achieved within 9 min with the number of theoretical plates of 7.27×10⁵ plates/m. The present system also performed good performance in the analysis of cytochrome C trypsin digest.
- Capillary electrophoresis,
- Electrospray ionization,
- Coaxial sheath flow,
- Hydrodynamic injection,
- Amino acid,
- Peptide analysis
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