Citation: ZHAO Yue-Zhen, XU Yang, GONG Can, JU Yu-Rui, LIU Zhao-Xin, XU Xu. Analysis of Small Molecule Compounds by Matrix-assisted Laser Desorption Ionization Mass Spectrometry with Fe₃O₄ Nanoparticles as Matrix[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(1): 103-112. doi: 10.19756/j.issn.0253-3820.201391 shu

Analysis of Small Molecule Compounds by Matrix-assisted Laser Desorption Ionization Mass Spectrometry with Fe₃O₄ Nanoparticles as Matrix

School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China

Corresponding author: XU Xu, xuxu3426@sina.com,xuxu@sit.edu.cn
Received Date: 5 July 2020
Revised Date: 1 November 2020

Fund Project: Supported by the National Natural Science Foundation of China (No.31671928) and the Natural Science Foundation of Shanghai, China (No.15ZR1440800).

The effect of matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) in analysis of small molecule compounds including amino acid, oligosaccharide and triglyceride was studied using nano triiron tetraoxide (Fe₃O₄) matrix. By comparing with other nanoparticle matrices and organic-inorganic binary matrices in the MALDI-MS analysis of D,L-pyroglutamic acid, D,L-aspartic acid, L-proline, L-phenylalanine, sucrose, raffinose, tripalmitin and triolein, the Fe₃O₄ nanoparticles were selected as matrix, and the effects of MALDI-MS experimental conditions on MS peak noise and signal intensity were investigated. As a result, Fe₃O₄ presented the characteristics of increasing the MS peak strength and reducing background noise. Using nano Fe₃O₄ matrix to cover sample, and setting MS acquisition parameters including polarity as positive and laser power of 70%, the results showed that the nano Fe₃O₄ was an ideal matrix for the detection of amino acids, oligosaccharide and triglycerides with good reproducibility and sensitivity. The MALDI-MS analysis of D,L-pyroglutamic acid, sucrose and triolein were performed using the nano Fe₃O₄ matrix and the in-spot repeatability RSDs were less than 3.2% and the spot-to-spot repeatability RSDs were less than 6.0%. The linear correlation coefficients (R²) between MS peak intensity and concentrations were determined as no less than 0.997 in the concentration range of 0.05-1.00 mg/mL, which showed potential quantitative power of the MALDI-MS analysis.
- Matrix assisted laser desorption ionization mass spectrometry,
- Triiron tetraoxide nanoparticles,
- Amino acid,
- Oligosaccharide,
- Triglyceride
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Analysis of Small Molecule Compounds by Matrix-assisted Laser Desorption Ionization Mass Spectrometry with Fe₃O₄ Nanoparticles as Matrix