Citation: XIAO Su-Ting, YAO Zheng, ZHOU Lin, YANG Pei-Hui. Real-time Monitoring of Adhesion between Oxidative Damaged-Erythrocytes and Endothelial Cells Based on Quartz Crystal Microbalance[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(11): 1550-1555. doi: 10.19756/j.issn.0253-3820.201335 shu

Real-time Monitoring of Adhesion between Oxidative Damaged-Erythrocytes and Endothelial Cells Based on Quartz Crystal Microbalance

College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China

Corresponding author: YANG Pei-Hui, typh@jnu.edu.cn
Received Date: 10 June 2020
Revised Date: 6 August 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21874057).

The investigation of the adhesion between red blood cells and vascular endothelial cells is of great significance to reveal the pathogenesis of cardiovascular disease. Under pathological conditions, the oxidative damage of red blood cells (RBCs) can cause down-regulating content of sialic acid combined with glycoprotein receptors on the cell membrane, and change the viscoelastic properties of the cells, lead to the enhancement of the adhesion of erythrocytes to vascular endothelial cells. In this work, a new method based on specific recognition of sialic acid and 3-aminophenylboronic acid integration with quartz crystal microbalance with dissipation (QCM-D) was developed for real-time monitoring the adhesion between damaged-erythrocytes and endothelial cells. Taking H₂O₂ oxidative damage to erythrocytes as a research model, the changes of the frequency and dissipation factor during cell adhesion process were monitored by QCM sensor, which was used to evaluate the degree of erythrocytes oxidative damage. The results showed that both the QCM frequency change and the dissipation factor decreased accordingly with the increase of H₂O₂ concentration. It revealed that the adhesion of erythrocytes was negatively correlated with the concentration of H₂O₂ and the degree of RBCs damage. Furthermore, endothelial cells were fixed on the sensing interface for simulating intravascular environment and their adhesion to red blood cells was monitored by QCM in real time. The results showed that the adhesion between cells increased with the increase of the degree of erythrocytes damage. Therefore, the strategy provided a simple, label free, highly sensitive and real-time method for the monitoring of cell adhesion and the evaluation of cells oxidative damage, which expended the application of QCM in the research of cell function in biological system.
- Erythrocytes,
- Oxidative damage,
- Endothelial cells,
- Adhesion,
- Quartz crystal microbalance
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