Citation: HOU Ji-Ting, WANG Bing-Ya, ZHENG Ling-Yun. Synthesis and Investigation of Fluorescent Probe for Peroxynitrite Detection with High Selectivity[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(11): 1502-1510. doi: 10.19756/j.issn.0253-3820.201299 shu

Synthesis and Investigation of Fluorescent Probe for Peroxynitrite Detection with High Selectivity

1.
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China;
2.
Analysis&Testing Center, Xinyang Normal University, Xinyang 464000, China

Corresponding author: HOU Ji-Ting, houjiting2206@163.com
Received Date: 24 May 2020
Revised Date: 23 June 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21807029) and the Nanhu Scholars Program for Young Scholars of Xinyang Normal University.

Peroxynitrite (ONOO^-) is one of the important reactive oxygen species in biosystems, and its effective detection has been attracting numerous attention. A fluorescent probe for ONOO^- detection was prepared via one-step reaction of 4-(diethylamino)salicylaldehyde and 6-methoxy tetralone. The optical response of the probe toward ONOO^- was investigated through UV and fluorescence spectra. The results showed that the probe was superior with the merits of high selectivity, superb sensitivity (the detection limit is 15.5 nmol/L), rapid response (within seconds), large Stokes shift (42 nm) and good water solubility. The 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay indicated an excellent biocompatibility of the probe (the survival rate of the cells was higher than 95% when the concentration of the probe was 20 μmol/L). Finally, the probe was successfully utilized for the fluorescence imaging of exogenous and endogenous ONOO^- in living cells.
- Fluorescent probe,
- Reactive oxygen species,
- Peroxynitrite,
- Cellular imaging
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