Citation: ZHENG An-Qi, HAO Ya-Nan, GUO Ting-Ting, SHU Yang, WANG Jian-Hua. Correlation of Fluorescence Imaging with Inductively Coupled Plasma-Mass Spectrometry for Quantification of Intracellular Copper Nanoparticles[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(10): 1359-1366. doi: 10.19756/j.issn.0253-3820.201270 shu

Correlation of Fluorescence Imaging with Inductively Coupled Plasma-Mass Spectrometry for Quantification of Intracellular Copper Nanoparticles

Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China

Received Date: 13 May 2020
Revised Date: 21 July 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21974018, 21727811) and the Fundamental Research Funds for the Central Universities (Nos. N2005015, N2005027).

A fluorescence imaging protocol was developed for the quantification analysis of intracellular glutathione-copper nanoparticles (GSH-CuNPs). After culturing MCF-7 cells with near infrared luminescent GSH-CuNPs, the fluorescence imaging of intracellular CuNPs was achieved at λ_ex/λ_em=559/(600-700) nm. The cells were then subjected to lysis and ultrafiltration to collect the GSH-CuNPs. The copper content in the collected GSH-CuNPs was quantified by inductively coupled plasma-mass spectrometry (ICP-MS). Thereafter, the fluorescence density in the imaging was correlated with the analytical data by ICP-MS with SPSS statistics analysis to develop a linear calibration between the fluorescence intensity and the intracellular content of GSH-CuNPs. The developed protocol here was applied to the investigation of GSH-CuNPs metabolism/transformation in live MCF-7 cells, and it was found that with incubation time of 50 min, the content of GSH-CuNPs decreased from 172.74 fg/cell to 18.66 fg/cell and the majority of GSH-CuNPs were metabolized and transformed into soluble copper species.
- Copper nanoparticles,
- Cell,
- Fluorescence imaging,
- Quantification,
- Inductively coupled plasma-mass spectrometry
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